噁喹酸、氟甲喹在鱼组织中残留量的检测方法研究  

Study on the Detection Method of Oxolinic Acid and Flumequine Residues in Fish Tissues

作　　者：仲锋[1] 董琳琳[1] 汪霞[1] 

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《中国兽药杂志》2002年第11期20-22,共3页Chinese Journal of Veterinary Drug

摘　　要：用高效液相色谱法，荧光检测器，同时检测鱼组织中恶喹酸，氟甲喹的残留量。用乙酸乙和为提取液，匀浆，离心，浓缩。HPLC条件：荧光检测器，激发波长325nm，发射波长369nm。流动相：0.02mol/L磷酸＋乙腈＋四氢呋喃（69＋16＋15）。恶喹酸，氟甲喹标准曲线线生范围10－500ng/mL，对带皮的鱼肉组织，进行3种浓度的回收率测定，其回收率在80％－10％之间，变异系数在11％以内，恶喹酸，氟甲喹的检测灵敏度为20ng/g。The high-performance liquid chromatography (HPLC) with fluorescence detector was used for the simul-taneous determination of oxolinic acid and flumequine residues in fish tissues. The drugs were extracted from tissueswith dried ethyl acetate, and the extract was concentrated to dry. The residue was dissolved in 0.02 mol/L phosphricacid. The HPLC separation was carried out on a C18 Luna (5m) column (150 mm×4.6 mm i.d.) with 0.02 mol/Lphosphoric acid + acetonitrile + tetrahydrofuran (69+16+15) as the mobile phase. A fluorescence detector was usedat an excitation wavelength of 325 nm and an emission wavelength of 369 nm. The calibration graphs were linear from10~500 ng/mL for oxolinic acid and flumequine. Average recoveries of two drugs from fish tissues fortified at level of20~300 ng/g were 80%~100%. Coefficients of variation were not more than 11%, and the detection limits were 20 ng/gfor oxolinic acid and flumequine.

关 键 词：恶喹酸 氟甲喹 鱼组织 残留量 检测方法 高效液相色谱 

分 类 号：S948[农业科学—水产养殖] TS254.7[农业科学—水产科学]