喉癌及癌旁组织中癌基因扩增的研究  

THE AMPLIFICATION OF C-myc, C-Ha-ras AND c-erb B_2 IN HUMAN LARYNGEAL CANCER AND LARYNX TISSUE SURROUDING THE TUMORS

作　　者：徐平西[1] 徐扬[1] 钱微[1] 郭志祥 

机构地区：[1]第四军医大学分子生物学研究室,西安710032 [2]空军沈阳医院耳鼻喉科,沈阳110042

出　　处：《癌症》1992年第5期335-337,共3页Chinese Journal of Cancer

基　　金：国家自然科学基金

摘　　要：东北地区是我国喉癌高发病区,为研究喉癌发生的机制,我们用打点杂交法对26例喉癌及其部分癌旁组织中c-myc、c-Ha-ras及c-erb B_2三种癌基因的扩增进行了观察。结果表明,三种癌基因在肿瘤组织中均有扩增;c-myc扩增率为84％(22/26),平均扩增了4倍;C—Ha—ras扩增率为61％(16/26),平均扩增3倍;c—erb B_2扩增率为50％(13/26),平均扩增2倍。癌旁组织中,三种癌基因扩增显著,其中c-Ha-ras的扩增高出肿瘤组织的5倍(P<0.01)。伴有肿瘤复发和转移的病例,其c-Ha-ras、c-erb B_2基因的扩增也有增高趋势。实验结果提示,不仅c-Ha-ras和c-erb B_2基因扩增与喉癌诱发、复发及转移密切相关,同时c-Ha-ras基因扩增的强度可作为追踪喉癌复发风险的线索。Very high frequency of laryngeal cancer occurs in northeast of China. To study the possible mechanism of its carcinogenesis, we have examined the amplification of C-myc, C-Ha-ras and C -erb B 2 with DNA dot blotting in human laryngeal cancer and the larynx tissue surrounding the tumors as compared to normal larynx tissue. All 3 oncogenes were amplified, about 4 folds in 22 of 26 cases (84%) for C-myc, 3 folds in 16 of 26 (61%) for C-Ha-ras and 2 folds in 13 of 26 (50%) for C-erb B 2 .Three oncogenes were also amplified in the tissuses surrounding the tumors. Interestingly, the amplification of C-Ha-ras in the tissues was higher than its adjacent tumor .(P<0.01) . The case with recurrence and metastasis showed strong tendency in amplification of C-Ha-ras.These observations indicate that the amplification of three oncogenes are nvolved in carcinogenesis of laryngeal cancer and that the tissue with higher amplification of C-Ha-ras possibly precede the formation of laryngeal cancer.The fact of remarkable C-Haras amplification could be useful to explore clinical predicators of disease recurrence.

关 键 词：基因扩增 喉肿瘤 癌基因 

分 类 号：R739.650.2[医药卫生—肿瘤]