Intergrin gene expression profiles of human hepatocellular carcinoma  

作　　者：Lian-Xin Liu Hong-Chi Jiang Wei-Hui Zhang An-Long Zhu Department of Surgery,the First Clinical College,Harbin Medical University,Harbin 150001,Heilongjiang Province,China Lian-Xin Liu Zhi-Hua Liu Jing Zhou Xiu-Qin Wang Min Wu National Laboratory of Molecular Oncology,Department of Cell Biology,Cancer Institute,Chinese Academy of Medical Science & Peking Union Medical College,Beijing 100021,China 

出　　处：《World Journal of Gastroenterology》2002年第4期631-637,共7页世界胃肠病学杂志（英文版）

基　　金：China Key Program on Basic Research,Grant Number:Z19-01-01-02;Chinese Climbing Project No.18;Youth Natural Scientific Foundation of Heilongjiang Province

摘　　要：AIM: To investigate gene expression profiles of intergringenes in hepatocellular carcinoma (HCC) through theusage of Atlas Human Cancer Array membranes, semi-quantitative reverse transcription polymerase chainreaction (RT-PCR) and Northern blot.METHODS: Hybridization of cDNA array membrane wasperformed with α 32P-labeled cDNA probes synthesizedfrom RNA isolated from hepatocellular carcinoma andadjacent non-cirrhotic liver. AtlasImage, which is asoftware specific to array, was used to analyze theresult. RT-PCR of 24 pairs specimen and Northern blotof 4 pairs specimen were used to confirm the expressionpattern of some intergrin genes identified by Atlasarrays hybridization.RESULTS: Among 588 genes spotted in membrane, 17genes were related to intergrin. Four genes were up-regulated, such as intergrin alpha8, beta1, beta7 andbeta8 in HCC. Whereas there were no genes down-regulated in HCC. RT-PCR and Northern blot analysisof intergrin beta1 gene gave results consistent withcDNA array findings.CONCLUSION: Investigation of these intergrin genesshould help to disclose the molecular mechanism of thecell adhesion, invasive and metastasis of HCC. A fewgenes are reported to have changed in HCC for the firsttime. The quick and high-throughout method of profilinggene expression by cDNA array provides us overviewof key factors that may involved in HCC, and may findthe clue of the study of HCC metastasis and moleculartargets of anti-metastasis therapy. The preciserelationship between the altered genes and HCC is amatter of further investigation.AIM:To investigate gene expression profiles of intergrin genes in hepatocellular carcinoma(HCC)through the usage of Atlas Human Cancer Array membranes,semi- quantitative reverse transcription polymerase chain reaction(RT-PCR)and Northern blot, METHODS:Hybridization of cDNA array membrane was performed with α 32P-labeled cDNA probes synthesized from RNA isolated from hepatocellular carcinoma and adjacent non-cirrhotic liver.AtlasImage,which is a software specific to array,was used to analyze the result.RT-PCR of 24 pairs specimen and Northern blot of 4 pairs specimen were used to confirm the expression pattern of some intergrin genes identified by Atlas arrays hybridization. RESULTS:Among 588 genes spotted in membrane,17 genes were related to intergrin.Four genes were up- regulated,such as intergrin alpha8,betel,bete7 and beta8 in HCC.Whereas there were no genes down- regulated in HCC.RT-PCR and Northern blot analysis of intergrin beta1 gene gave results consistent with cDNA array findings. CONCLUSION:Investigation of these intergrin genes should help to disclose the molecular mechanism of the cell adhesion,invasive and metastasis of HCC.A few genes are reported to have changed in HCC for the first time.The quick and high-throughout method of profiling gene expression by cDNA array provides us overview of key factors that may involved in HCC,and may find the due of the study of HCC metastasis and molecular targets of anti-metastasis therapy.The precise relationship between the altered genes and HCC is a matter of further investigation.

关 键 词：肝癌 整合素 基因表达 RT-PCR 作用机制 

分 类 号：R735.7[医药卫生—肿瘤]