The anabolic effects of recombinant human growth hormone and glutamine on parenterally fed,short bowel rats  

作　　者：Yan Gu Zhao-Han Wu Department of General Surgery,Zhongshan Hospital,Fudan University,Shanghai 200032,China 

出　　处：《World Journal of Gastroenterology》2002年第4期752-757,共6页世界胃肠病学杂志（英文版）

摘　　要：AIM: To evaluate the metabolic effects associated withadministration of rhGH and/or Gln in parenterally fed,short-bowel rats.METHODS: Forty SD rats subjected to 75 % intestinalresection and maintained with parenteral nutritionwere randomly divided into 4 groups as follows: -rhGH,-Gln; -rhGH, +Gln; +rhGH, -Gln; +rhGH, +Gln.Bodyweight and nitrogen balance were evaluated daily. After6 days of PN, rats were killed, various organs weredissected and weighted, the carcasses were used foranalysis of body composition. Serum GH and IGF-1 weredetermined by RIA method.RESULTS: Weight loss in rats with rhGH (17.4±12.8 g)and rhGH+Gln (23.8±3.5 g) was significantly less thanrats with PN alone (29.6±6. 9 g) and rats with Gln-supplemented PN (31.85±12.8 g), P＜0.05. Theaccumulated NB in rats with rhGH (1252.9±294.3 mgN/d) and rhGH+Gin (1261.7±85.5 mg N/d) wassignificantly greater than those with PN alone (704.8±379.0 mg N/d) and with Gln-supplemented PN(856.7±284.4 mg N/d), P＜0.05. The absolute weightof gastrocnemius muscle in rats with rhGH (2683.9±341.6 mg) and rhGH+Gln (2579.1±359.5 mg) wasgreater than those with PN alone (2176.3±167.1 mg)and with Gln-supplemented PN (2141.9±353.6 mg).Although the absolute weight of remnant smallintestine itself was not significantly different in 4experimental groups, the weight/length of thesegments was greater in rats with rhGH and/or Gln(48.7±5.5, 52.7±4.1 and 67.4±5.3 respectively) thanthose with PN alone(47.8±5.0), there were synergisticeffects between rhGH and Gln in improvement of theweight/length of remnant small intestine, P＜0.05.Analyses of body carcass composition showed that ahigher percentage of carcass weight as protein and alower percentage of carcass weight as fat wereoccurred in rats with rhGH (20.8±4.0,6.0±2.6) andrhGH+Gin(21.3±2.4,4.4±1.5) than those with PNalone (16.4±2.4,9.2+3.7) and with Gln-supplementedPN (17.8±3.0,6.3±2.0), rhGH had significant effectson alteration of body composition, P＜0.05. Serum GHand IGF-1 concentration in rats with rhAIM:To evaluate the metabolic effects associated with administration of rhGH and/or Gin in parenterally fed, short-bowel rats. METHODS:Forty SD rats subjected to 75 % intestinal resection and maintained with parenteral nutrition were randomly divided into 4 groups as follows:-rhGH, -Gin;-rhGH,+Gin;+rhGH,-Gin;+rhGH,+Gin.Body weight and nitrogen balance were evaluated daily.After 6 days of PN,rats were killed,various organs were dissected and weighted,the carcasses were used for analysis of body composition.Serum GH and IGF-1 were determined by RIA method. RESULTS:Weight loss in rats with rhGH(17.4±12.8 g) and rhGH+GIn(23.8±3.5 g)was significantly less than rats with PN alone(29.6±6.9 g)and rats with Gin- supplemented PN(31.85±12.8 g),P<0.05.The accumulated NB in rats with rhGH(1252.9±294.3 mg N/d)and rhGH+GIn(1261.7±85.5 mg N/d)was significantly greater than those with PN alone(704. 8±379.0 mg N/d)and with Gin-supplemented PN (856.7±284.4 mg N/d),P<0.05.The absolute weight of gastrocnemius muscle in rats with rhGH(2683.9 ±341.6 rag)and rhGH+GIn(2579.1±359.5 mg)was greater than those with PN alone(2176.3±167.1 mg) and with GIn-supplemented PN(2141.9±353.6 mg). Although the absolute weight of remnant small intestine itself was not significantly different in 4 experimental groups,the weight/length of the segments was greater in rats with rhGH and/or Gin (48.7±5.5,52.7±4.1 and 67.4±5.3 respectively)than those with PN alone(47.8±5.0),there were synergistic effects between rhGH and Gin in improvement of the weight/length of remnant small intestine,P<0.05. Analyses of body carcass composition showed that a higher percentage of carcass weight as protein and a lower percentage of carcass weight as fat were occurred in rats with rhGH(20.8±4.0,6.0±2.6)and rhGH+GIn(21.3±2.4,4.4±1.5)than those with PN alone(16.4±2.4,9.2±3.7)and with Gin-supplemented PN(17.8±3.0,6.3±2.0),rhGH had significant effects on alteration of body composition,P<0.05.Serum GH and IGF-1 concentration in rats with rhGH(5.221±0.8 and

关 键 词：重组人生长激素 谷氨酸 胃肠外营养 短肠鼠 体重 

分 类 号：R574[医药卫生—消化系统]