培养软骨对胫骨生长影响的实验研究  

Effect of Cultured Cartilages on Tibial Growth

作　　者：王建[1] 杨志明[1] 解慧琪[1] 秦廷武[1] 

机构地区：[1]第三军医大学新桥医院骨科,重庆400037

出　　处：《中国矫形外科杂志》2002年第12期1189-1191,共3页Orthopedic Journal of China

基　　金：国家基础研究发展规划项目 (G19990 54 30 8)

摘　　要：目的 :探讨培养软骨植入胫骨干骺端缺损后的组织学改变 ,以及对胫骨生长的影响。方法 :自 3周龄兔关节面分离软骨细胞 ,经离心管培养 2周形成软骨。于 1 6只 1 2周龄新西兰兔右侧胫骨近端生长板下方 1 .5mm处造成边缘性缺损 ,移植培养软骨。左侧胫骨未作手术为正常对照。分别于术后 6、1 2周进行X线摄片和组织学检查。结果 :6周时胫骨近端生长板变窄 ,其下方 6mm处形成薄层软骨或不规则软骨块 ,缺乏生长板柱状细胞排列结构。大量新骨形成和植入区皮质骨增厚。 1 2周时生长板基本闭合 ,干骺端结构正常 ,无软骨。双下肢X线摄片显示双侧胫骨生长无差异 (P >0 .0 5)。结论 :培养软骨植入干骺端可以维持组织性质 。Objective:To investigate histological changes of cultured cartilages and influence on tibial growth by allograft into tibial metaphyseal defects.Methods:Chondrocytes were isolated from joint surfaces of rabbits aged 3 weeks and formed cartilages by cultivation in centrifuge tubes for 2 weeks.Cultured cartilages were allografted into peripheral defects of proximal tibiae of 16 New Zealand rabbits aged 12 weeks,which were 1.5mm below growth plates.Left tibia remained untreated as control.Histological and radiographic examinations were respectively performed at 6,12 weeks after surgery.Results:At 6 weeks after operation,the growth plates of proximal tibiae became narrower.One thin layer of cartilage or irregular cartilaginous block formed in the tibial metaphysis 6mm below growth plate,which lacked cellular columnar organization of normal growth plate. A great deal of new bone formed,and cortical bone became wide on the graft site.At 12 weeks,the growth plates of proximal tibiae were nearly closed and metaphyseal structure was normal without cartilages.There was not developmental difference of tibiae between the bilateral limbs according to radiography.Conclusion:Cultured cartilages may keep their histological nature after allograft into metaphyses,but they lack structural characteristic and growth ability of growth plate.

关 键 词：培养软骨 胫骨生长 实验研究 生长板 移植 组织工程 

分 类 号：R-331[医药卫生]