抗肿瘤坏死因子单抗对内毒素血症家兔临界氧提取率的影响  

Effects of anti-tumor necrosis factor monoclonal antibody on the critical oxygen extraction ratio of endotoxemic rabbits

作　　者：朱明光[1] 章志红[2] 韩历萍[2] 吴兴裕 

机构地区：[1]中国人民解放军第四军医大学吉林军医学院应用生理学教研室,吉林132013 [2]中国人民解放军第四军医大学空军医学系

出　　处：《中国急救医学》2002年第11期621-622,共2页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金资助项目 (No .3 9170 3 5 1)

摘　　要：目的　观察抗肿瘤坏死因子单抗对内毒素血症大鼠组织氧提取率的影响。方法　以内毒素静脉注射法复制动物模型 ,以电磁血流量计和血气分析仪测量并计算氧运输量、氧耗量和氧提取率。结果　内毒素组临界氧运输量、氧耗量分别为 (14 1± 3 8)mL·min-1·kg-1和 (8 7± 1 7)mL·min-1·kg-1,显著高于对照组 [分别为 (9 8± 3 4 )mL·min-1·kg-1,P <0 0 1和 (7 2± 1 3)mL·min-1·kg-1,P <0 0 5 ],临界氧提取率 (0 5 7± 0 0 6 )显著低于对照组 (0 80± 0 19,P <0 0 1)。抗体保护组临界氧运输量、氧耗量及氧提取率 [分别为 (10 0± 3 7)mL·min-1·kg-1,(7 5± 1 6 )mL·min-1·kg-1和 0 82± 0 2 1]与对照组无显著差异 (P >0 0 5 )。无关抗体组上述指标与内毒素组无显著差异 (P >0 0 5 )。结论　肿瘤坏死因子在内毒素所致的家兔氧提取能力下降中起重要介导作用 ,应用中和性单克隆抗体可以提高组织氧提取率。Objective To observe the influence of anti-tumor necrosis factor monoclonal antibody on the tissue oxygen extraction ratio of rabbits.Methods The animal model was made by venous endotoxin injection,the electromagnetic blood flow meter and blood-gas analyser were used to measure and account oxygen delivery,oxygen consumption and oxygen extraction ratio.Results The critical oxygen delivery and oxygen consumption in endotoxin group〔(14.1±3.8)mL·min -1·kg -1 and (8.7±1.7) mL·min -1·kg -1,respectively〕 was significantly higher than control group [(9.8±3.4)mL·min -1·kg -1,P<0.01 and (7.2±1.3)mL·min -1·kg -1, P<0.05 respectively],the critical oxygen extraction ratio(0.57±0.06) was notably lower than control group (0.80±0.19,P<0.01);No notable difference was found between anti-TNF McAb group [(10.0±3.7) mL·min -1·kg -1,(7.5±1.6)mL·min -1·kg -1 and 0.82±0.21 respectively] and control group (P>0.05).The difference between uncorrelated McAb group and endotoxin group was not significant(P>0.05).Conclusion Tumor necrosis factor acts as a pivotal mediator in endotoxin-induced rabbit oxygen extractin ratio defect,and the use of anti-TNF McAb can improve the oxygen extraction ratio of endotoxemic rabbit.

关 键 词：内毒素血症 氧提取率 抗肿瘤坏死因子单克隆抗体 家兔 临界氧提取率 

分 类 号：R363.2[医药卫生—病理学]