肿瘤坏死因子与内毒素在重症急性胰腺炎中的作用  

The Effect of Tumor Necrosis Factor and Endotoxin in Severe Acute Pancreatitis

作　　者：张平平[1] 张瑞玲[2] 杜金郎[1] 王金龙[1] 

机构地区：[1]内蒙古医学院附属医院普外科,内蒙古呼和浩特010050 [2]中国人民解放军第253医院检验科,内蒙古呼和浩特010051

出　　处：《内蒙古医学杂志》2002年第5期394-396,共3页Inner Mongolia Medical Journal

摘　　要：目的 :探讨肿瘤坏死因子、内毒素在重症急性胰腺炎 (SAP)中的作用。方法 :制成SAP模型。分别于术后 6h、12h、2 4h、4 8h测定血浆TNFα、内毒素、淀粉酶水平 ,并对胰腺超微结构和病理变化进行观察。结果 :SAP组犬血浆TNFα、内毒素、淀粉酶水平明显高于对照组 ,以发病后 12h、2 4h最为明显 (P <0 0 1～P<0 0 5 ) ,胰腺坏死灶及大量皂化 ,光镜胰腺细胞模糊不清 ,腺泡有大量片状坏死出血 ,部分胞核溶解消失 ,坏死胰腺周围可见大量中性白细胞浸润。结论 :TNFα 和内毒素是导致急性胰腺炎发生的重要因素 ,抑制TNFα 和内毒素的产生 ,可缓解SAP的发展。Objective:To investigate the effect of tumor necrosis factor-α(TNF α) and endotoxin in the acute severe pancreatitis.Method:After SAP model induced in dogs,plasma levels of TNF α?endotoxin and amylase were measured at 6h?12h?24h?48h?after operation.Pathological changes and ultrastructure of pacreatitis were observed.Results:The plasm levels of TNF α?endotoxin and amylase were significantly higher in SAP group than in the control group,especially on 12h?24h?(P<0.01～P<0.05).The tissue demages of pancreas were observed.The pancreatic cells observed by microscope were demaged.There were hemorrhagic necrosis.Part of nucleus were karyolysis.There were much leukocyts around the necrosis tissue of pancreas.Conclusion:The TNF α and endotoxin were important factors in the development of severe acute pancreaetitis.The development of severe acute pancreaetitis may be alleviated by inhibitng the production of TNF α and endotoxin.

关 键 词：肿瘤坏死因子 内毒素 重症急性胰腺炎 SAP 动物模型 

分 类 号：R576[医药卫生—消化系统]