邻羧基苯基重氮氨基偶氮苯分光光度法测定粮食中的微量汞  

Spectrophotometric Determination of Mercury in Cereals with o-carboxybenzenediazoaminobenzene

作　　者：杨玉玲[1] 丁耀 李在均 

机构地区：[1]南京经济学院食品科学工程系,南京210003 [2]内蒙古通辽北国家粮食储备中转库,通辽028021 [3]江南大学,无锡214036

出　　处：《中国粮油学报》2002年第5期64-66,共3页Journal of the Chinese Cereals and Oils Association

摘　　要：本文通过研究汞与显色剂邻羧基苯基重氮氨基偶氮苯的显色反应 ,找到了一种测定粮食中汞的新方法。粮食样品中的汞经过消化提取和净化处理后 ,在常温下 ,pH为 10的缓冲溶液中与邻羧基苯基重氮氨基偶氮苯反应生成红色络合物 ,此络合物的最大吸收波长位于 5 10nm ,它的表观摩尔吸光系数为 1.7×10 5L .mol- 1.cm- 1,汞的浓度在 0～ 8μg/ 2 5mL范围内与吸光度之间存在线性关系 ,相关系数为 0 .9975。用此方法测粮食中微量汞比国家标准方法二法 (双硫腙法 )更准确。A novel spectrophotometric method for determination of mercury in cereals based on the reaction of o-carboxybenzenediazoaminobenzene with mercury was developed.After digestion of cereal sample and proper purification,mercury,at room temperature,reacts with o-carboxybenzenediazoaminobenzene in borate buffer solution (pH about 10) to form a red complex compound.The compound has a maximum absorption peak at 510nm,The apparent molar absorptivity of the complex is 1.7×10 5L.mol -1 .cm -1 .The method showed excellent linearity over the concentration range of 0-8μgHg 2+ /25mL with the linear correlation co-efficient of 0.9975.The method was proved to be more accurate and precise than the second method of national standard (the method of dithizono).

关 键 词：邻羧基苯基重氮氨基偶氮苯 分光光度法 测定 粮食 汞 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程] S379.1[轻工技术与工程—食品科学与工程]