沙克沙豆凝集素的分离纯化及其性质的研究  

Purification and Characteristics of Sakesa Lectin

作　　者：程昕[1,2] 唐富良[1,2] 刘守安 王公禹[1,2] 姚益新 陈华堂[1,2] 

机构地区：[1]安徽医科大学生化教研室 [2]安徽医科大学分子生物学研究室

出　　处：《安徽医科大学学报》1992年第1期5-10,共6页Acta Universitatis Medicinalis Anhui

摘　　要：用PBS抽提、硫酸铵分段沉淀及猪甲状腺球蛋白—Sepharose 4B亲和层析可从腰点沙克沙(phaseolus vulgaris L)中分离得沙克沙凝集素,达到电泳纯。SDS—聚丙烯酰胺凝胶电泳得出一条带,分子量为30.26×10~3u,表明该凝集素由单一亚基组成。其含糖量为5%。该凝集素能凝集兔、小鼠、鸡、人A、B、O红细胞,其凝集作用可被猪甲状腺球蛋白明显抑制。该凝集素可有效地促进淋巴细胞转化和分裂;体外实验表明,它可凝集Sp 2/0骨髓瘤细胞。Sakesa Lectin was obtained by extraction with PBS, precipitation with ammonium sulfate, and affinity absorption on porcine thyroglobulin-Sepharose 4B from a seed of Phaseolus vulgaris L. SDS-polyacrylamide electrophoresis with or without 1% mercaptoethanol gave one band with molecular weights of 30.26×10~3U, indicating that the lectin consisted of identical subunits. Its total carbohydrate content was 5.0%. The lectin agglutinated rabbit, mouse, chicken and human ABO blood cell, and the activity of this lectin was inhibited by porcine thyroglobulin. Sakesa Lectin was a strong mitogen for human peripheral lymphocytes. The experiments in vitro showed that Sakesa Lectin could agglutinate mouse spleen lymphocytes and Sp2/0 myeloma cells.

关 键 词：凝集素 沙克沙豆 色谱法 肿瘤细胞 

分 类 号：R394－33[医药卫生—医学遗传学]