用斑点核酸杂交检测临床“合格血”HBV-DNA  

USING DOT-HYBRIDIZATION TO EXAMINE HBV-DNA IN TRANSFUSIBLE BLOOD

作　　者：余元勋[1] 罗运满 丁子清[2] 

机构地区：[1]安徽省医学科学研究所 [2]安徽省立医院输血科

出　　处：《安徽医学》1992年第3期46-47,共2页Anhui Medical Journal

摘　　要：本文对235份临床血库常规检查后的“合格血”用地高辛-HBV-DNA斑点核酸杂交法检测HBV-DNA,共8份阳性,阳性率3.4%。其中7份每斑点含HBV-DNA 1～10微微克,另1份为10～50微微克.讨论了本方法对加强输血质控的意义,并对现行的输血员筛检方法提出改进建议。Using digoxin-HBV-DNA probe to make a dot DNA hybridization, we examined 235 blood samples that had no HBsAg in the blood to be detected by ELISA method. We called these blood 'transfusible blood' which we found 8 positive samples (positive rate 3.4%). In 7 samples each dot (50μl serum)had HBV-DNA 1-10pg, and in another sample the dot had HBV-DNA 10-50 Pg. We discussed that the new method could help to improve the blood transfers'screening and the quality control of blood transfusion.

关 键 词：血液 HBV-DNA 检测 

分 类 号：R446.11[医药卫生—诊断学]