地高辛抗血清拮抗大鼠缺血再灌注引起的脑损伤(英文)  

Anti-digoxin antiserum protects brain against injuries caused by ischemia-reperfusion in rats

作　　者：柯永胜[1] 胡爱群[2] 张根葆[2] 

机构地区：[1]皖南医学院弋矶山医院心脏内科 [2]皖南医学院生理学教研室,安徽芜湖241001

出　　处：《中国神经科学杂志》2002年第4期719-723,共5页

基　　金：安徽省自然科学基金 (0 10 43 90 2 );安徽省教委基金 (99j10 2 19;2 0 0kj2 5 6)资助项目

摘　　要：研究地高辛抗血清对缺血再灌注脑损伤的拮抗作用 ,采用了大鼠全脑缺血再灌注模型 ,比色法检测脑匀浆液中超氧化物歧化酶 (SOD)活性、丙二醛 (MDA)含量、脑细胞膜Na+ K+ 交换ATP酶活性、血清肌酸激酶(CK)含量 ;用放射免疫法检测脑匀浆液中内洋地黄素含量。结果发现 ,全脑缺血再灌注导致脑组织SOD活性和ATP酶活性显著下降 ,脑组织MDA水平、内洋地黄素水平和血清CK水平显著升高。地高辛抗血清能改善由于脑缺血再灌注所造成的SOD、ATP酶活性的下降以及MDA、CK和内洋地黄素水平的升高。结果表明 ,地高辛抗血清对脑缺血再灌注脑损伤具有保护作用 ,其作用机制与减轻脂质过氧化、促进自由基的清除以及改善脑能量代谢有关。这些作用可能是通过拮抗内洋地黄素的作用而实现的。The present study examined whether the protective effects of anti digoxin antiserum on rat brain injury existed. Global cerebral ischemia reperfusion model in rats was employed. Spectrophotometric assay methods were used to measure the activity of superoxide dismutase (SOD), ATPase activity, content of malondialdehyde (MDA) in rat brain and content of creatine kinase (CK) in rat serum,and radioimmunoassay method was used to measure the content of endoxin in rat brain homogenate. The results showed that global cerebral ischemia reperfusion led to the decrease in SOD activity and ATPase activity, and the increase in MDA, endoxin and serum CK levels. Anti digoxin antiserum could inhibit the decrease in SOD and ATPase activities and the increase in MDA, CK and endoxin levels caused by ischemia reperfusion in rat brain.Anti digoxin antiserum showed a protective action against the ischemia reperfusion induced injury in rat brain by reducing lipid peroxides, scavenging free radicals and improving the energy metabolism. These effects may be introduced by antagonizing endoxin.

关 键 词：脑损伤 地高辛抗血清 超氧化物歧化酶 丙二醛 肌酸激酶 内洋地黄素 Na^+-K^+-交换ATP酶 全脑缺血再灌注 动物实验 放射免疫法 

分 类 号：R743[医药卫生—神经病学与精神病学]