阻塞性黄疸患者一氧化氮合酶的表达及其与肝损害的关系  

Nitric oxide synthase expression in the patients with obstructive jaundice and relation to hepatic injury

作　　者：张剑[1] 李茂德[1] 何生[1] 

机构地区：[1]贵阳医学院附院,550004

出　　处：《贵州医药》2002年第11期990-991,共2页Guizhou Medical Journal

摘　　要：目的研究一氧化氮合酶在阻塞性黄疸患者肝组织中表达及其与肝损害的关系。方法采用免疫组化方法检测 5 0例阻塞性黄疸 (OJ)患者和 30例对照肝脏组织诱生型一氧化氮合酶 (iN OS)的表达 ,计算机图像分析仪对肝脏组织坏死面积进行检测。结果 (1)iNOS在 86 %的OJ肝脏的组织中阳性表达 ,在对照组肝脏组织中不表达。 (2 )肝脏组织坏死面积和iNOS表达强度呈显著正相关(P <0 0 1)。结论iNOS表达与阻塞性黄疸患者肝损害有关 ,并在其中起重要作用。Objective To study inducible nitric oxide synthase (iNOS) protein expression in the patients with obstructive jaundice and its relation to hepatic injury. Methods. Thirty patients with obstructive jaundice (OJ) were selected as experimental group and thirty patients with cholelithiasis as control group. Tissue section from 50 OJ patients were examined immunohistochemically for protein expression of iNOS and hepatic tissue necrosis area (HTNA) was calculated by image analysis system at H&E stain. Results 1.Positive iNOS immunostaining was detected in 86% of OJ patients. The iNOS was not detected in control group. 2. There were positive relationships between the HTNA and the degree of positive iNOS expression in OJ patients. Conclusion The excessive expression of iNOS may play an important role in hepatic injury of patients with obstructive jaundice.

关 键 词：阻塞性黄疸 一氧化氮合酶 肝脏损害 免疫组化 

分 类 号：R575[医药卫生—消化系统]