河南食管癌高、低发区居民食管癌前病变和癌组织维甲酸受体β的表达  

Alterations of retinoid acid receptor (RARβ) expression in esophageal precancerous and cancerous lesions from the subjects at high and low-incidence areas for esophageal cancer in Henan

作　　者：岳文彬[1] 高珊珊[1] 刘宾[2] 白永敏[1] 范宗民[1] 齐义军[1] 刘刚[1] 郭花芹[1] 张延瑞[3] 冯常炜[4] 李吉林 焦新英 王立东[1] 

机构地区：[1]郑州大学医学院癌症研究室,郑州450052 [2]首都医科大学同仁医院消化内科,北京100730 [3]河南省人民医院消化内科,郑州450003 [4]郑州大学第二附属医院消化内科,郑州450003 [5]林州市姚村食管癌医院,林州456592

出　　处：《郑州大学学报（医学版）》2002年第6期766-768,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家杰出青年科学基金 3 0 0 2 5 0 16;河南省高校创新人才工程基金;河南省医药卫生创新人才工程基金资助项目

摘　　要：目的 :探讨食管癌高、低发区人群正常食管上皮和各级癌前病变组织中维甲酸受体 β(RARβ)蛋白表达变化的特征及其与形态变化之间的关系。方法 :食管癌高发区患者 70例 ,低发区患者 77例 ,采用卵白素 生物素 辣根过氧化氢酶复合物 (ABC)法 ,检测高、低发区居民食管组织中RARβ蛋白表达。 结果 :RARβ免疫阳性反应主要定位于细胞膜和细胞浆。高、低发区居民食管正常上皮和各级病变组织中均有不同程度的免疫阳性反应 ,从正常→基底细胞过度增生 (BCH)→间变 (DYS)→鳞状细胞癌 (SCC) ,高发区免疫阳性率分别为 16 %、2 7%、31%、6 0 % ,低发区分别为 13%、38%、4 0 %、6 6 %。随SCC分化程度升高 ,RARβ阳性反应百分率亦呈升高趋势 ;高发区居民高、中、低分化SCC组织中RARβ的免疫阳性率为 95 %、73%、2 5 % ,低发区分别为 95 %、77%、39% ,两地区间分化程度相似的SCC组织中RARβ阳性率相比差异无统计学意义 (P >0 .0 5 )。但是 ,高发区低分化与中、高分化SCC组织免疫反应阳性率相比差异有统计学意义 (P <0 .0 1) ,与低发区SCC结果相似。结论 :RARβ蛋白表达发生于食管癌变的各个阶段 ,食管癌高、低发区存在相似的改变 ,其蛋白表达的阳性率随SCC分化程度升高而显著升高 ,提示RARβ改变可能与食管癌细胞的分?Aim: To characterize the changes of retinoic acid receptor β (RARβ) expression in esophageal precancerous and cancerous lesions from the subjects at high and low incidence areas for esophageal cancer in Henan. Methods: Immunohistochemistry (ABC) and histopathological methods were used to determine the expression of RARβ. Results: Positive immunoreactivity of RARβ was observed in the normal tissues and the lesions at different degrees of severity on the subjects from the high incidence area (HIA) and low incidence area (LIA). The positive immunoreactivity was chiefly located at the cell membrane and cytoplasma. From normal to basal cell hyperplasia (BCH) to dysplasia (DYS) to squamous cell carcinoma (SCC), the RARβ positive immunostaining rates were 16%,27%,31% and 60%, respectively in HIA,and 13%,38%, 40% and 66%, respectively in LIA. From poorly to moderate,and well differentiated SCC, the positive rates of RARβ were 25%,73% and 95% in HIA, respectively and 39%,77% and 95% in LIA, respectively.Both in HIA and LIA, significant difference occurred between poorly to moderate and well differentiated SCC ( P <0.01). Conclusion: The present results indicate that the alternations of RARβ may be correlated with the differentiation of esophageal SCC.

关 键 词：河南 食管癌 低发区 高发区 居民 食管癌前病变 癌组织 维甲酸受体 

分 类 号：R735.1[医药卫生—肿瘤]