一株具抗肿瘤活性的北极细菌的筛选及分子鉴定  

The Screening and Molecular Identification of Arctic Marine Psychrophilic Microorganism with Anti-tumor Function

作　　者：曾润颖[1] 李根[2] 林昱[2] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]国家海洋局第三海洋研究所海洋生物工程重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2002年第6期800-803,共4页Journal of Xiamen University：Natural Science

基　　金：中国首次北极科学考察研究课题;国家科技部基础性工作项目 (G 99A 15 )资助

摘　　要：在中国首次北极科学考察期间 ,从北极海域采集了水体 ,沉积物等样品 ,从中分离得到一批嗜冷细菌 ,采用MTT法对这些北极海洋细菌进行了细胞毒活性物质的筛选 ,得到一株具有细胞毒活性的菌株 .采用A number of psychrophilic microorganisms were isolated from arctic sea water and sediment samples collected during the China First Arctic Investigation cruise. A strain with cell toxicity was isolated by MTT screening model which was used to identify anti-tumor active material. The genera of the strain was identified based on comparison and phylogenetic analysis of 16S rDNA gene sequence.

关 键 词：抗肿瘤活性 北极细菌 MTT分析法 细胞毒活性物质 分子鉴定 菌株筛选 海洋微生物 

分 类 号：R284[医药卫生—中药学] Q938.8[医药卫生—中医学]