口服三种形式眼镜蛇毒的急性毒性及对肝癌移植瘤的抑瘤作用  

Acute toxicity test of three Cobra Venom preparations and their inhibitory effect on transplanted hepatoma in mice

作　　者：孙鹏[1] 徐辰 任先达 刘建军[2] 李晓红 

机构地区：[1]暨南大学药学院药代动力学教研室,广东广州510632 [2]暨南大学药学院药理学教研室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2002年第4期1-4,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国务院侨办重点课题科研基金资助项目 (98- 33)

摘　　要：目的 :了解口服 3种形式眼镜蛇毒的急性毒性及对肝癌的在体抑瘤作用。方法 :按Bliss法设计急性毒性实验 ,蛇毒兔血清、原毒和经酸热处理后蛇毒分别一次性给小鼠灌胃 ,观察其急性毒性。移植小鼠腹水型肝癌于小鼠右腋皮下 ,应用以上 3种形式蛇毒连续灌胃 9d ,每天给药量以最大耐受量 (LD1)的 1/3,计算其抑瘤率。结果 :小鼠口服上述 3种形式蛇毒的LD50 及 95 %可信限分别为 5 4 10mg·kg- 1(46 5 0～ 6 2 90mg·kg- 1)、5 2 94mg·kg- 1(42 0 7～ 6 6 6 3mg·kg- 1)和 97 4 5mg·kg- 1(83 6 2～ 113 5 6mg·kg- 1)。 3种形式蛇毒 2次抑瘤实验的抑瘤率 :蛇毒兔血清冻干粉为35 5 %、32 9% ,原毒为 2 2 9%、2 0 3% ,经酸热处理后蛇毒为 2 5 3%、2 1 3%。结论 :眼镜蛇毒原毒的抗肿瘤作用在蛋白变性或消化吸收后仍然得以保留 。Aim: To study the acute toxicity of three Cobra Venom(CV) preparations and their inhibitory effect on transplanted hepatoma in mice. Methods: The acute toxicity test was designed accor ding to the Bliss method. Mice were orally given different dose of three CV preparations. The acute toxi city was observed. After the 24 hours of the inoculation of mouse hepatoma H 22, three CV preparations were given orally in a dose of the 1/3 of LD 1 for 9 days. The inhibitory rates of tumor were calculated from its weight change. Results: LD 50 and 95% Conf.Lim.of three preparations were respectively 5 410 mg·kg -1 (4 650～6 290 mg·kg -1), 52.94 mg·kg -1 (42.07～66.63 mg·kg -1) and 97.45 mg·kg -1 (83.62～113.56 mg·kg -1). In two experiments, the tumor weight inhibitory rates of the serum preparations were 35.5%?32.9%. Those of the others were 22.9%?20.3% and 25.3%?21.3%. Body weights of mice were markedly increased after the drug administration in all groups. Conclusion: After the CV was denatured or digested, its anticancer activity was still remained. The toxicity of the serum preparations was lowered than that of the others, but the tumor inhibitory effect more remarkable.

关 键 词：眼镜蛇毒 急性毒性 肝癌移植瘤 抑瘤作用 

分 类 号：R735.7[医药卫生—肿瘤]