玫瑰红绿海葵触手cDNA表达文库的构建和初步分析  

The Construction of cDNA Expression Library from the Tentacles of Sagartia rosea

作　　者：刘文华[1] 王义良[1] 陈慧萍[1] 姜孝玉[1] 涂洪斌[1] 卫剑文[1] 彭文烈[1] 徐安龙[1] 

机构地区：[1]中山大学生命科学学院生物化学系,国家863计划海洋生物功能基因组开放实验室,广州510275

出　　处：《生物工程学报》2002年第6期749-753,共5页Chinese Journal of Biotechnology

基　　金：国家高新技术海洋领域 86 3项目 (No .819 0 6 0 6 )& (No.2 0 0 1AA6 2 6 0 10 )资助~~

摘　　要：A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART TM protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART TM protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.

关 键 词：玫瑰红绿海葵 触手 cDNA表态文库 构建 

分 类 号：Q782[生物学—分子生物学]