转基因小鼠中外源基因遗传及表达稳定性的研究  

The Study on the Stable Inheritance and Expression of Foreign Gene in Transgenic Mice

作　　者：颜景斌[1] 肖艳萍[1] 方彧聃[1] 奚鹰[1] 黄文英[1] 黄英[1] 

机构地区：[1]上海市儿童医院上海医学遗传研究所,上海200040

出　　处：《生物工程学报》2002年第6期758-760,T001,共4页Chinese Journal of Biotechnology

基　　金：国家"8 6 3"高技术项目基金资助 (No .2 0 0 1AA2 130 11);上海市现代生物与新药产业发展基金资助 (No .9943190 4);上海市卫生局青年基金资助 (No.0 0 4Y10 )~~

摘　　要：Two transgenic mouse strains , in which the expression of human factor IX (hFIX) in the milk were different significantly, were bred, and the foreign gene integration as well as the content of hFIX in the milk were detected by PCR, Southern blot, FISH and ELISA, respectively. The results showed that approximately 50% offsprings were transgenic positive. Foreign gene integrated in mouse chromosomes was intact. The hFIX expression of each mouse in the same strain was different, the content of hFIX in the milk was (43.32±5.41)μg/mL in FIX-33 transgenic strain and (1.16±0.45)μg/mL in FIX-124 transgenic strain. Meanwhile, the hFIX gene expression between the two strains was different remarkably (P<0.01). We conclude that the characteristics of inheritance and expression in the founder were able to be transferred to their offsprings stably.Two transgenic mouse strains , in which the expression of human factor IX (hFIX) in the milk were different significantly, were bred, and the foreign gene integration as well as the content of hFIX in the milk were detected by PCR, Southern blot, FISH and ELISA, respectively. The results showed that approximately 50% offsprings were transgenic positive. Foreign gene integrated in mouse chromosomes was intact. The hFIX expression of each mouse in the same strain was different, the content of hFIX in the milk was (43.32±5.41)μg/mL in FIX-33 transgenic strain and (1.16±0.45)μg/mL in FIX-124 transgenic strain. Meanwhile, the hFIX gene expression between the two strains was different remarkably (P<0.01). We conclude that the characteristics of inheritance and expression in the founder were able to be transferred to their offsprings stably.

关 键 词：转基因小鼠 外源基因 遗传 表达稳定性 

分 类 号：Q819[生物学—生物工程]