抗人卵巢癌×抗人CD3双特异性单链抗体的构建、表达及复性研究  

Construction and Expression of an Anti-human Ovarian Carcinoma×Anti- human CD3 Bispecific Single-chain Antibody and its Refolding Studies

作　　者：方敏[1] 赵瑞[2] 李骅[1] 蒋欣[1] 尹长城[1] 林晴[1] 黄华 

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100101 [2]西北农林科技大学生命科学院,杨凌712100

出　　处：《高技术通讯》2002年第11期47-50,共4页Chinese High Technology Letters

基　　金：863计划 (863 10 2 0 9 0 4 0 1)资助项目

摘　　要：构建了抗人卵巢癌×抗人CD3双特异性单链抗体 (scBsAb) ,在大肠杆菌中得到表达。用稀释复性 ,缓慢透析复性和凝胶过滤层析复性三种方法对scBsAb表达形成的包涵体进行复性研究表明 ,凝胶过滤层析复性能有效抑制蛋白的聚集。ELISA检测显示 ,复性后的scBsAb具有较高活性 ,能与相应抗原特异结合。An anti human ovarian carcinoma×anti human CD3 bispecific single chain antibody(scBsAb) is constructed and expressed in the E. coli strain GI724 as inclusion body. Three different refolding procedures had been sused to perform renaturation of the scBsAb. Direct dilution and gradual dialysis both leaded to aggregation of the refolded proteins. However, size exclusion chromatography performing reneturaton could supprss protein aggregation. ELISA tests demonstrate that the refolded scBsAb has good activity, can bind to its target antigens with specificity.

关 键 词：抗人卵巢癌 抗人CD3 双特异性单链抗体 包涵体 复性 基因表达 肿瘤免疫治疗 

分 类 号：R737.31[医药卫生—肿瘤]