动脉吻合术后弹性纤维的扫描电镜观察  

SCANNING ELECTRONIC MICROSCOPE OBSERVATION OF ELASTIC FIBER OF ANASTOMOSED ARTERY

作　　者：邱全光[1] 余汇洋[1] 可金星[2] 郭光金[1] 

机构地区：[1]第三军医大学外科应用解剖与手术学教研室,重庆400038 [2]第三军医大学中心实验室电镜室,重庆400038

出　　处：《中国修复重建外科杂志》2002年第6期371-373,共3页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：重庆市基础研究基金资助项目 ( 19980 36 )

摘　　要：目的　研究动脉吻合术后弹性纤维的构筑。　方法　在手术显微镜下将 Wistar大鼠 60只右侧股动脉横行切断后行端端吻合术 ,于手术后 3、7、1 4、2 1、30和 90天切取吻合侧动脉及对侧动脉 ,经 1 0 %中性甲醛溶液固定 ,将动脉段纵行中间切开 ,置于 90 %甲酸中 ,45℃消化 96小时 ,液氮速冻 ,置真空蒸发器中干燥 2 4小时 ,取出真空镀金后在扫描电镜下观察弹性纤维在动脉吻合术后的重构。　结果　动脉吻合术后 3、7天吻合口中心弹性纤维缺失 ,术后 1 4～90天 ,新生弹性纤维由新生内膜部位向吻合口中心延续。弹性纤维的重构分为 :静止期、增生期、重建期。　结论　动脉壁的弹性纤维在术后经历了重建过程 ,30天弹性纤维的形态结构趋于正常。Objective To observe the architecture of elastic fiber of anastomosed artery. Methods The right femoral arteries of 60 Wistar rats were cut off transversely and end to end anastomosis were performed. On the 3rd, 7th, 14th, 21st, 30th and 90th days after operation , the anastomosed artery segments were harvested and fixed by 10% formalin. After routine processed, the architecture of elastic fiber of anastomosed artery was observed under scanning electronic microscope and was compared with that of normal artery. Results On the 3rd and 7th days after anastomosis, there was no the elastic fiber in the middle of the anastomosed area. From 14 to 90 days after anastomosis, the newborn elastic fiber connected the anastomosed area. The reconstruction of elastic fiber could be divided into quiescent stage, proliferation stage, and rebuilding stage. Conclusion The reconstruction of elastic fiber occurs after arterious anastomosis and newborn elastic fiber originates from endoarterious layer. The structure of elastic fiber can return to normal 30 days after anastomosis.

关 键 词：动脉吻合术 弹性纤维 扫描电镜 动脉损伤 实验研究 

分 类 号：R654.3[医药卫生—外科学]