截短型转化生长因子-βⅡ型受体在皮肤成纤维细胞的过度表达  

OVEREXPRESSION OF TRUNCATED TYPE Ⅱ TRANSFORMING GROWTH FACTOR-β RECEPTOR IN DERMAL FIBROBLASTS

作　　者：刘伟[1] 蔡泽浩[1] 商庆新[1] 钱云良[1] 曹谊林[1] 

机构地区：[1]上海第二医科大学附属第九人民医院整复外科,上海200011

出　　处：《中国修复重建外科杂志》2002年第6期404-407,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：上海市科委重大课题资助项目 ( 0 0 JC14 0 32 )

摘　　要：目的　研究截短型转化生长因子 -β(TGF-β) 型受体在皮肤成纤维细胞的过度表达对 TGF-β1 自分泌的抑制作用。　方法　组织块法体外培养正常皮肤成纤维细胞 ,加入重组人 (rh) TGF- β1 (5ng/ ml)或含截短型 TGF- β 型受体基因的重组腺病毒 (50 pfu/ cell) ,并用 Northern印迹观察其对 TGF-β1 的基因调控。　结果　 rh TGF-β1 能上调TGF-β1 和 I型前胶原的基因表达。截短型 TGF-β 型受体的过度表达能减少细胞 TGF-β1 的基因表达。　结论　截短型TGF- β 型受体的过度表达可以通过阻断 TGF- β1 的信号转导来抑制皮肤成纤维细胞 TGF- β1 的自分泌。Objective To study the effect of overexpression of truncated type Ⅱ TGF β receptor on transforming growth factor β 1(TGF β 1 )autoproduction in normal dermal fibroblasts. Methods In vitro cultured dermal fibroblasts were treated with recombinant human TGF β 1(rhTGF β 1) (5ng/ml) or recombinant adenovirus containing truncated type Ⅱ TGF β receptor gene (50 pfu/cell). Their effects on regulating gene expression of TGF β 1 were observed with Northern blotting. Results rhTGF β 1 up regulated the gene expression of TGF β 1 and type I procollagen. Overexpression of truncated receptor Ⅱ down regulated the gene expression of TGF β 1. Conclusion Overexpression of the truncated TGF β receptor Ⅱ decreases TGF β 1 autoproduction via blocking TGF β receptor signal. The results may provided a new strategy for scar gene therapy.

关 键 词：截短型 Ⅱ型受体 成纤维细胞 转化生长因子-Β 自分泌 信号转导 基因治疗 瘢痕增生 组织纤维化 

分 类 号：R363[医药卫生—病理学]