2-氨基甾体对慢性粒细胞白血病细胞系K562细胞增殖的抑制作用  

Inhibitory effect of a novel 2-aminosteroid on the proliferation of K562 leukemia cells

作　　者：李琼[1] 何群[1] 

机构地区：[1]中南大学湘雅医学院血液生理研究室,长沙410078

出　　处：《湖南医科大学学报》2002年第5期405-408,共4页Bulletin of Hunan Medical University

基　　金：国家自然科学基金(3 9970 3 16)

摘　　要：目的 :观察 2 氨基甾体 (MPZ)对慢粒白血病细胞系K5 6 2细胞增殖的作用。方法 :采用形态观察、液体培养、集落培养及MTT实验检测MPZ对K5 6 2细胞增殖的影响 ,并观察其形态学变化。结果 :液体培养 6d后 ,10 - 8和10 - 5mol·L- 1 MPZ对K5 6 2细胞的抑制率分别为 4 6 %和 83%。集落培养 8d后 ,10 - 8和 10 - 5mol·L- 1 MPZ对K5 6 2细胞的抑制率分别为 5 2 %和 10 0 %。MTT实验检测表明 ,10 - 8和 10 - 5mol·L- 1 MPZ处理K5 6 2细胞 5d后其抑制率分别为2 9%和 88%。结论 :MPZ(10 - 8～ 10 - 5mol·L- 1 )能明显抑制K5 6 2细胞的增殖 。Objective To evaluate the inhibitory effects of a novel 2 aminosteroid (MPZ) on the proliferation of K562 leukemia cells. Methods The effects of MPZ on the proliferation of K562 leukemia cells were in vitro studied in terms of morphology observation, cell number numeration both in liquid and in semi solid colony cultures and UV absorption in the MTT assay. Results It was verified that the inhibition of K562 leukemia cells was 46% with the 10 -8 mol·L -1 MPZ treatment and 83% with the 10 -5 mol·L -1 MPZ treatment respectively in the liquid culture for 6 days. The inhibition of K562 cells in the colony assay on day 8 was 52% and 100% implemented respectively by 10 -8 and 10 -5 mol·L -1 MPZ. In the MTT assay, K562 cells were inhibited by 29% and 88% respectively after the treatment with 10 -8 and 10 -5 mol·L -1 MPZ for 5 days. Conclusion The proliferation of K562 leukemia cells was inhibited by MPZ (10 -8 ～10 -5 mol·L -1 ) in a dose dependent manner.

关 键 词：抑制作用 K562细胞系 培养的细胞 增殖 2-氨基甾体 慢性粒细胞白血病 

分 类 号：R733.71[医药卫生—肿瘤]