阴蒂海绵体中磷酸二酯酶5表达及淫羊藿甙对cGMP浓度的影响  

Expression of phosphodiesterase 5 in clitoral corpus cavernosum and effect of icariin on cGMP levels in vitro

作　　者：乔梁[1] 辛钟成[1] 付杰[1] 刘武江[1] 林桂亭[1] 陈山[2] 

机构地区：[1]北京大学第一医院泌尿外科北京大学泌尿外科研究所,100034 [2]首都医科大学附属北京同仁医院泌尿外科

出　　处：《中华泌尿外科杂志》2002年第11期670-672,共3页Chinese Journal of Urology

基　　金：国家自然科学基金 (3 0 0 70 92 7) ;北京市自然科学基金(70 12 0 2 3 ) ;中华医学基金人才基金 (文 0 47)

摘　　要：目的　探讨阴蒂海绵体中磷酸二酯酶 5表达及淫羊藿甙对cGMP浓度的影响。　方法　通过反转录聚合酶链反应技术 (RT PCR)检测兔阴蒂海绵体中PDE5mRNA的表达 ;12 5I放射免疫法测定不同浓度淫羊藿甙对阴蒂海绵体内PDE5酶底物cGMP浓度的影响 ,西地那非作为阳性对照 ,分别计算EC50 。　结果　阴蒂海绵体组织中有PDE5的表达 ,淫羊藿甙可明显提高阴蒂海绵体内的cGMP浓度 ,具有显著的浓度依赖性。　结论　淫羊藿甙对阴蒂海绵体平滑肌细胞内cGMP水平的影响可能是通过PDE5发挥作用 ,与NO cGMP信号通路有关。Objective To investigate the expression of phosphodiesterase 5 (PDE5) in clitoral corpus cavernosum and effect of icariin on cyclic GMP (cGMP) levels in vitro. Methods The presence of mRNA coding for PDE5 in clitoral corpus cavernosum was detected by means of RT PCR analysis.The effect of icariin with various concentrations on cGMP levels,which is the target of PDE5 in vivo,was measured by 125 I radioimmunoassay with sildenafil as positive control. EC 50 of icarrin and sildenafil was calculated. Results There was a lane around 800 bp on the agar electrophoresis of the production of RT PCR,which was as long as we designed according to cDNA sequence of PDE5.In the presence of SNP,icariin increased cGMP concentrations in rabbit clitoral corpus cavernosum with increasing dose. Conclusions The results support the proposal that icariin may influence cGMP level by NO cGMP signaling pathway as an inhibitor of PDE5.

关 键 词：阴蒂 海绵体 磷酸二酯酶5 淫羊藿甙 CGMP 浓度 女性 性功能障碍 治疗 

分 类 号：R699[医药卫生—泌尿科学]