热诱导颊癌细胞凋亡与Bcl-x_L蛋白表达的关系  

The relationship between apoptosis induced by hyperthermia and the expression of Bcl-x_L in BcaCD885 cells

作　　者：何永文[1] 边莉[1] 漆雪梅[1] 刘流[1] 

机构地区：[1]昆明医学院第一附属医院口腔颌面外科,650032

出　　处：《现代口腔医学杂志》2002年第6期494-495,共2页Journal of Modern Stomatology

基　　金：云南省自然科学基金资助项目 (编号 :1999C0 0 0 7R)

摘　　要：目的　探讨热诱导BcaCD885细胞凋亡与Bcl-xL 蛋白表达变化的关系。方法　 4 3 C 4 0min水浴加温诱导BcaCD885细胞产生凋亡。采用流式细胞仪 (FCM) -DNA及抗体检测技术分别测定细胞凋亡率及Bcl-xL蛋白表达量 ,观测热诱导颊癌细胞凋亡过程中Bcl-xL 蛋白表达的动态变化 ,探讨二者间的关系。结果　凋亡组内Bcl-xL 蛋白表达量明显低于对照组 (P <0 .0 5 ) ,凋亡率与Bcl-xL 蛋白表达水平存在负相关关系 (r =- 0 .89,P<0 .0 5 )。结论　Bcl-xLObjective To study the relationship between mechanism of hyperthermia-induced apoptosis and the expression of Bcl-x L protein in BcaCD885 cells.Methods After inducing apoptosis through hyperthermia with 43C 40min,we studied the apoptotic rate and the expression of Bcl-x L protein through FCM-DNA and antibody analysis technique respectively.Results After hyperthermia,there was down-regulation of Bcl-x L protein in apoptotic groups(P< 0.05 ).The correlation coefficient r between the apoptotic rate and the expression of Bcl-x L protein was - 0.89 (P< 0.05 )?Conclusion Hyperthermia acting as an external signal may down-regulate expression of Bcl-x L protein and then induces apoptosis in BcaCD885 cells.Bcl-x L is an antagonist to hyperthermia-induced apoptosis.

关 键 词：热诱导 颊癌 细胞凋亡 BCL-XL 蛋白表达 BCACD885细胞 流式细胞术 抗体检测 

分 类 号：R739.81[医药卫生—肿瘤]