白细胞介素-18在急性移植物抗宿主病发病机制中的作用  

Role of Interleukin-18 in Pathogenesis of Acute Graft-Versus-Host Disease

作　　者：居小萍[1] 王健民[1] 侯军[1] 冯曹波[1] 童书鹏[1] 章卫平[1] 

机构地区：[1]第二军医大学附属长海医院血液科,上海200433

出　　处：《中国实验血液学杂志》2002年第5期452-454,共3页Journal of Experimental Hematology

基　　金：国家自然科学基金 (编号 39870 710 );上海市卫生系统百人计划基金 (编号98BR0 2 9)资助项目

摘　　要：为了研究异基因外周血造血干细胞 (allo PBSCT)移植期细胞因子白细胞介素 1 8(IL 1 8)与急性移植物抗宿主病 (aGVHD)的关系 ,以探讨IL 1 8在aGVHD发病中的作用 ,对 2 6例恶性血液病患者进行了allo PBSCT ,并采用双夹心酶联免疫吸附法 (ELISA)检测移植前及移植后aGVHD发生时患者外周血单个核细胞分泌的IL 1 8浓度。结果表明 :移植患者中 1 0例发生Ⅰ度GVHD和 5例发生Ⅲ -Ⅳ度GVHD ,发生aGVHD患者分泌的IL 1 8浓度显著高于未发生aGVHD患者 ,并且IL 1 8浓度与aGVHD的严重程度成正相关 ,与感染的发生无关。结论 :IL 1To investigate the relationship between interleukin 18(IL 18) and human acute graft versus host disease(aGVHD), 26 patients undergoing allogeneic peripheral blood stem cell transplantation(allo PBSCT) were included in this study. IL 18 secreted by peripheral blood mononuclear cells(MNCs) was mearsured by enzyme linked immunosorbent assay(ELISA) before transplantation and during aGVHD. The results showed that grade I GVHD and grades Ⅲ Ⅳ GVHD developed in 10 and 5 cases, respectively. The levels in the supernatants of MNCs from patients with aGVHD were significantly higher than those in the cases without aGVHD. The levels of IL 18 were correlated with the severity of aGVHD. It is concluded that IL 18 plays an important role in the development of aGVHD.

关 键 词：异基因外周血干细胞移植 急性移植物抗宿主病 白细胞介素—18 白血病 发病机制 

分 类 号：R733.7[医药卫生—肿瘤]