检测白血病细胞增殖力的MTS/pms比色分析法的建立  

Development of MTS/pms Colorimetric Assay in the Proliferation of Leukemic Cells

作　　者：陈秀生[1] 方铁兰[1] 蔡瑞波[2] 郭桂兰[2] 

机构地区：[1]陕西省人民医院老年病研究中心,西安710068 [2]西安交通大学第一医院血液病中心,西安710061

出　　处：《中国实验血液学杂志》2002年第5期438-440,共3页Journal of Experimental Hematology

摘　　要：为了探讨建立一种更为快速、安全、灵敏的白血病细胞增殖力的检测方法 ,本研究应用HL 60和K562细胞可转化MTS/pms形成水溶性待测产物 ,其光密度值在 492nm波长下半自动测定的特点 ,研究了MTS/pms比色分析法取代MTT和INT用于白血病细胞增殖力的检测。结果表明 ,只有活的白血病细胞才能转化MTS/pms形成待测产物 ,且白血病细胞数与所测光密度值 (OD)之间具有良好的相关性 (r =0 .963) ;与MTT和INT法相比较 ,MTS/pms比色分析法中所形成的最终待测产物为水溶性 ,省略了上清液去除和加入有机溶剂的步骤。结论 :MTS/pms比色分析法对白血病细胞增殖力的检测更具有快速、安全、准确。In order to establish a new more rapid, safe and sensitive colorimetric assay for the proliferation of leukemic cells, MTS/pms has been developed. This automated colorimetric assay is based on the characteristic of viable and metabolically active leukemic cells to cleave MTS/pms into a water soluble product whose optical density is determined at 492 nm by an automated microtiter plate reader photometer. The results indicated that only active leukemic cells cleaved MTS/pms into product measured, and dead cells did not reduce MTS/pms. A linear relationship were found between the viable cell number and optical density of MTS/pms cleaved by HL 60 and K562 cell(r=0.963). Compared with MTT and INT assays, the reduced product of MTS/pms is water soluble. It is concluded that MTS/pms colorimetric assay is more rapid, accurate and sensitive for the bioassay of proliferation of leukemic cells.

关 键 词：白血病细胞 HL—60细胞 K562细胞 细胞增殖 MTS/pms比色分析法 检测方法 光密度值 

分 类 号：R733.7[医药卫生—肿瘤] R446.[医药卫生—临床医学]