四色荧光标记抗体在白血病免疫分型中的应用及意义  

The Application of 4-Color Fluorescence Labeling Antibodies and Its Significance in Immunophenotyping for Leukemia by Flow Cytometer

作　　者：刘艳荣[1] 于弘[1] 常艳[1] 陈珊珊[1] 

机构地区：[1]北京大学血液病研究所,北京100044

出　　处：《中国实验血液学杂志》2002年第5期423-427,共5页Journal of Experimental Hematology

摘　　要：为探讨多参数流式细胞术对白血病免疫分型的方法及意义 ,本研究自行设计了一系列 4种荧光标记的单克隆抗体组合 ,对 88例急性白血病患者骨髓或外周血进行流式细胞仪免疫表型分析。应用本组抗体组合可对T ALL和B ALL及AML M1 -M7患者做出诊断 ,并可诊断形态学难以辨认的AML M0 ,及一般免疫分型法较难辨认的单核细胞白血病。应用 4组四色荧光标记的抗体组合 ,共含 1 3种抗体 ,既可将 90 %以上的白血病患者分为ALL或AML ,并可鉴别急性白血病的主要亚型 ,及反映血细胞的分化成熟度。结论 :四色荧光抗体组合可用较少的抗体 ,特异地对白血病细胞进行多参数免疫分型分析 。To prove the significance of 4 color fluorescence labeling flow cytometric analysis for leukemia immunophenotyping, bone marrow and peripheral blood samples from 88 cases with acute leukemia were analyzed by means of flow cytometry with 4 color fluorescence labeling monoclonal antibodies(McAbs). More than 90% of cases could be classified into acute myeloid, T or B lymphocytic leukemia by staining with 13 different kinds of monoclonal antibodies within 4 tubes of combination of antibodies. AML M 2-M 7 could also be identified simultaneously. Another one tube of combination of 4 McAbs could be used for further subtyping of those who were undetermined by the above 4 tubes. Patients with AML M 0/M 1 which may be negative of myeloperoxidase(MPO) in cytochemistry can be identified by labeling with both membrane and cytoplasmic antibodies. In conclusion, the accuracy of leukemia immunphenotyping has been improved by 4 color flow cytometry analysis.

关 键 词：白血病 免疫分型 四色荧光标记抗体 多参数分析 多参数流式细胞术 

分 类 号：R733.7[医药卫生—肿瘤] R730.[医药卫生—临床医学]