银染端粒重复序列扩增法检测肝癌模拟肝穿组织端粒酶活性的研究  

Detection of Telomerase Activity in Simulative Fine-Needle Biopsy Tissues of Hepatocellular Carcinoma by Non-Radioisotopic Silver Staining Telomeric Repeat Amplification Protocol

作　　者：卫立辛[1] 范瑞芳[1] 杨庆[1] 贾凤岐[1] 王维锋[1] 郭亚军[1] 吴孟超[1] 

机构地区：[1]第二军医大学东方肝胆外科医院肿瘤免疫和基因治疗中心,上海市200438

出　　处：《中国肿瘤临床》2002年第11期772-774,共3页Chinese Journal of Clinical Oncology

基　　金：国家重点基础研究发展规划项目(973项目)基金资助(编号:G2000057001)

摘　　要：目的:探讨细针穿刺肝活检组织行端粒酶活性检测用于肝细胞癌(HCC)诊断的可行性。方法:HCC共28例,每例HCC肿瘤切除后立即行肝肿瘤模拟细针穿刺。应用非放射性同位素银染的端粒重复序列扩增法(TRAP),分别对28例模拟肝穿组织和28例肿瘤切取组织行端粒酶活性检测。结果:模拟肝穿组织与肿瘤切取组织的端粒酶检测结果完全一致,阳性率均为82%(23/28)。结论:模拟肝穿组织与肿瘤切取组织在HCC端粒酶活性检测方面具有同等的效果。该结果提示,B超引导下经皮肝穿刺活检行端粒酶活性检测在HCC的诊断、治疗效果评价和预后的判断方面具有一定的应用前景。Objective:To explore the feasibility of detection of telomerase activity in fine-needle biopsy tissues of hepatocellular carcinoma (HCC).Methods:Simulative fine-needle biop-sies were performed in28HCC nodules immediately after surgical resection.Telomerase activity of28fine-needle biopsy specimens and28incisional biopsy specimens were detected by non-ra-dioiso topic silver staining telomeric repeat amplification protocol(TRAP).Results:No difference was found between fine-needle biopsy specimens and incisional biopsy specimens with the same telomerase-positive rate(82%)in both groups.Conclusion:Both methods have the same effect for detection of telomerase activity,suggesting that detection of telomerase activity in tumorous tissues obtained by percutaneous fine-needle liver biopsy under the guidance of B-ultrasound should be feasible for diagnosis,assessment of curative effect and judgement of prognosis of HCC.

关 键 词：肝细胞癌 活组织检查 针吸 端粒酶 HCC 诊断 银染端粒重复序列扩增法 

分 类 号：R735.7[医药卫生—肿瘤] R730.[医药卫生—临床医学]