c-erbB-2多克隆抗体的制备及特性分析  

Preparation of Anti-cerbB-2 Polyclonal Antibody and Its Effects on Human Breast Cancer Cells

作　　者：顾斐[1] 邹强[1] 倪泉兴[1] 徐永华[2] 

机构地区：[1]复旦大学附属华山医院普外科,上海200040 [2]中国科学院上海细胞生物学研究所,上海200032

出　　处：《复旦学报（医学版）》2002年第6期447-450,共4页Fudan University Journal of Medical Sciences

摘　　要：目的　制备针对表皮生长因子受体 (c erbB 2 )细胞表面受体的抗体 ,不仅可阻止生长信号的传递 ,从而抑制肿瘤生长 ,还可通过抗体介导的细胞毒作用杀伤肿瘤细胞 ,具有重要的临床治疗应用价值。方法　通过重组表达获得了纯化的c erbB 2基因产物———融合蛋白GST c erbB 2 ,用此抗原制备兔抗血清 ,经rProteinGAgarose和GlutathioneSepharose 4B亲和层析柱纯化获得IgG型抗c erbB 2蛋白的多克隆抗体。对此抗体进行了细胞表面特异识别、体外肿瘤细胞生长抑制等方面的功能研究。结果　抗体可特异性识别重组表达蛋白、细胞表面c erbB 2抗原 ,并与之结合 ;抗体对c erbB 2阳性的人乳腺癌细胞SK BR 3有特异性的杀伤作用 ,且呈剂量相关 ,而对c erbB 2阴性的人乳腺癌细胞MCF 7则无明显作用。结论　c erbB 2抗体通过其对c erbB 2阳性细胞的特异识别和生长抑制 ,有广阔的临床应用前景。Purpose: c-erbB-2 is an attractive target for the delivery of therapeutic agents. Preparing antibody binding the ligand of c-erbB-2 can not only block the pathway of growth factors but also induce antibody dependent cell-mediated cytotoxity. Methods: c-erbB-2 was highly expressed in E. coli BL21(DE3) and purified. The c-erbB-2 protein was used as antigen to get IgG polyclonal antibody. The specificity and efficiency of the antibody were analyzed. Results: The antibody thus prepared can inhibit growth of SK-BR-3 cells lines, which are c-erbB-2 positive; but has no effect on MCF-7 cell lines, which are c-erbB-2 negative. Conclusions: The c-erbB-2 antibody may be beneficial to cancer therapy by specific recognization and tumor cells growth inhibition.

关 键 词：C-ERBB-2 多克隆抗体 制备 特性分析 乳腺癌细胞 

分 类 号：R737.9[医药卫生—肿瘤]