nm23-H1基因对舌癌细胞株化疗敏感性的影响体外实验  

The Influence of nm23 - H1 to Chemo - sensitivity in Oral Carcinoma Cell Lines in Vitro.

作　　者：陈绍维[1] 李唐新[2] 陈伟辉[3] 

机构地区：[1]中山大学附属第二医院口腔科,广东广州510120 [2]中山大学光华口腔医学院 [3]福建医科大学附属协和医院

出　　处：《广东牙病防治》2002年第4期245-247,共3页Journal of Dental Prevention and Treatment

基　　金：国家自然基金;39870746

摘　　要：目的 通过nm23-H1的转化及导入BcaCD85细胞株,观察nm23-H1对BcaCD885细胞株化疗敏感性的影响。方法 完成细胞培养和基因转染后,MTT法观察nm23-H1对BcaCD885化疗敏感性影响。结果 转染前BcaCD885细胞株对ADM、5-FU、MTX的化疗敏感性无显著差异。但转染后的BcaCD885细胞株对CDDP明显增敏。结论 nm23-H1可能通过特异性地影响细胞内的能量交换过程来达到对CDDP化疗增敏的效果。Objective To find out whether nm23 - H1 could affect the chemo- sensitivity of BcaCD885 cell lines by transfecting nm23 - H1 into the BcaCD885 cell lines. Methods Nm23 - HI was transfected into BcaCD885 cell lines and the change of chemo - sensitivity was assessed by MTT. Result The transfected BcaCD885 cell line is more sensitive to CDDP. Conclusion Nm23 - H1 can enhance chemo- sensitivity to CDDP significantly perhaps by affecting the exchange of the energy in cells.

关 键 词：NM23-H1基因 舌癌细胞株 化疗敏感性 体外实验 基因转染 细胞培养 

分 类 号：R739.86[医药卫生—肿瘤]