诺氟沙星在胶束体系中的荧光分析方法  

FLUOROPHOTOMETRIC ANALYSIS OF NORFLOXACIN IN A MICELLAR SYSTEM

作　　者：徐莉英[1] 蔡蕍[1] 

机构地区：[1]上海应用技术学院化工系,上海200235

出　　处：《理化检验（化学分册）》2002年第10期487-488,共2页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：对诺氟沙星在胶束体系中荧光特性进行了研究 ,发现十二烷基硫酸钠 (SDS)对诺氟沙星有较强的增敏作用。经分析研究 ,当诺氟沙星的浓度在 0 .0 3～ 0 .2 0 μg·ml- 1范围内 ,其荧光强度与浓度线性关系良好 ,检出限为 0 .0 3μg·ml- 1,回收率为 95 .0 %～ 98.3%。It was found that in a buffer solution of pH 5.5, the addition of an appropriate amount of sodium dodecyl sulfate (SDS) to the solution of norfloxaxin, showed a remarkable enhancement of the fluorescence intensity of norfloxacin. A further study proved that norfloxacin can be determined by fluorophotometry based on this micellar reaction system. Linear relationship was kept between the fluorescence intensities and the norfloxacin concentrations in the range of 0.03～0.20μg of norfloxacin per ml of solution. The detection limit and the range of recoveries of this method were found to be 0.03μg·ml -1 and 95.0%～98.3% respectively.

关 键 词：荧光分析 荧光光度法 诺氟沙星 胶束体系 抗菌药物 十二烷基硫酸钠 荧光增敏 

分 类 号：R978.1[医药卫生—药品] R919[医药卫生—药学]