乳腺癌KAI1基因表达及其临床病理意义  

The expression and clinicopathological significance of KAI1 gene in breast cancer

作　　者：高蓓[1] 何震宇[1] 张萍[1] 

机构地区：[1]南京医科大学第二附属医院普外科,江苏南京210011

出　　处：《河北医学》2002年第10期879-882,共4页Hebei Medicine

摘　　要：目的 :探讨乳腺癌KAI1表达及其临床病理意义。方法 :采用免疫组织化学和半定量逆转录多聚酶链反应 (RT -PCR)。检测 83例乳腺癌标本中KAI1蛋白和mRNA的表达并与临床病理资料比较分析。结果 :免疫组化的阳性率 4 8.19% (40 83)和阴性率 5 1.81% (43 83) ,RT -PCR的阳性率4 1.17% (35 83)和阴性率 5 7.83% (48 83)。结论 :免疫组化和RT -PCR的结果基本一致 (P >0 .0 5 )。并与乳腺癌的转移呈负相关。在Ⅲ、Ⅳ期以及发生淋巴结等转移的病人中 ,KAI1的表达明显下调 (P <0 .0 1)。Objective: To investigate the expression and clinicopathological significance of the KAI1 metastasis suppressor gene in breast cancer . Methods: The protein and mRNA expression of KAI1 gene were examined respectively by immunohischemistry and half-quantitative reverse transcriptase PCR (RT-PCR) methods in 83 patients with breast cancer. In addition , these findings were compared with the clinicopathological data. Results: Paralleled KAI1 mRNA levels. The positive and negative rate of KAI1 expression were 48.19% (40/83) ,51.81% (43/83) respectively by immunohischemistry and 42.17% (35/83) , 57.83% (48/83) respectively by RT-PCR . Conclusion : KAI1 protein levels were consistant basicolly with the KAI1 mRNA levels(P>0.05). KAI1 protein levels were inversely correlated with the metastatic patential of breast cancer.It obvious down-reguloted when the patients with Ⅲ?Ⅳ stages or lymphoid metastasis (P<0.01).

关 键 词：乳腺癌 KAI1基因 临床病理 免疫组织化学 逆转录多聚酶链反应 基因表达 

分 类 号：R737.9[医药卫生—肿瘤]