中效原理在顺铂、5-氟脲嘧啶、长春新碱对人卵巢癌细胞株(SKOV_3)相互作用中的应用  

The Interaction Between Cisplatin,5-Fluorouracil and Vincristine on Human Ovarian Cancer Cell line

作　　者：车艺 汤为学[2] 郑维萍[2] 

机构地区：[1]重庆职工医学院病理生理教研室,重庆400050 [2]重庆医科大学病理生理教研室,重庆400016

出　　处：《实用妇产科杂志》2002年第6期364-365,共2页Journal of Practical Obstetrics and Gynecology

摘　　要：目的 :体外观察顺铂、5 氟脲嘧啶、长春新碱配伍对人卵巢癌细胞株 (SKOV3)的相互作用。方法 :采用MTT比色法利用中效原理判定两药合用的效果。结果 :三种药物单用及合用时随着剂量增加其效应也增加 ;顺铂与 5 氟脲嘧啶合用时产生协同作用 ;顺铂与长春新碱合用时产生拮抗作用 ;5 氟脲嘧啶与长春新碱合用时 ,小于中效浓度时产生协同作用 ,大于中效浓度时产生拮抗作用。结论 :两种药物合用时可以产生协同或拮抗作用 ,而且效应大小与两药浓度比例有关。Objectives:To evaluate the cytotoxic effects of Cis,5 Fu and VCR on human ovarian cancer cell line(SKOV 3).Methods: The median effect principle was used to inves tigate theintera chion between two drugs by MTT assay.Results: Cytotoxic effects of each drug was enhanced with increased of drug concentration.The interaction of the Cis and 5 Fu was synergism,but that of the Cis and VCR was antagonism.Conclusion:The interaction of the two cytotoxic drugs was synergism or antagonism.The ratios of two drugs concentration was related to their cytotoxis effects.

关 键 词：顺铂 5-氟脲嘧啶 长春新碱 卵巢癌 抗癌药物联合应用 中效原理 抗癌药物敏感试验 MTT比色法 

分 类 号：R737.31[医药卫生—肿瘤]