吴茱萸汤醇提各组分止呕活性的研究  

Anti-emetic Effect of Ethanol Extract from "Wuzhuyu Broth

作　　者：张婷[1] 王敏伟[2] 陈思维[2] 

机构地区：[1]中国科学院上海药物研究所,上海200031 [2]沈阳药科大学药理系,辽宁沈阳110016

出　　处：《中国中药杂志》2002年第11期862-866,共5页China Journal of Chinese Materia Medica

摘　　要：目的 :对吴茱萸汤醇提各组分止呕活性的研究和机制初步探讨。方法 :采用硫酸铜诱导家鸽呕吐实验 ,小鼠胃排空实验 ,ACh和 5 HT作用的离体大鼠胃条实验来确定其中具有止呕活性的组分 ,并观察有效组分对ACh ,5 HT ,组胺累积浓度 效应曲线的影响。结果 :5 0 %醇洗脱液和 70 %醇洗脱液有十分显著性的止呕效应 ,且副作用较小 ,对ACh ,5 HT ,组胺累积浓度 效应曲线均有一定的作用。结论 :5 0 %醇洗脱液和 70 %醇洗脱液含有的止呕活性成分较多 ,5 0 %和 70 %醇可以从吴茱萸汤中提取较多止呕活性成分 ,止呕作用可能与拮抗ACh ,5 HT ,组胺受体有关。Objective:To investigate the anti-emetic effect of ethanol extract from ' WuZhuYu broth' and its mechanism. Method:Three experiments were carried out test which extract has anti-emetic activity, such as CuSO 4-induced pigeon's emetic response, gastric emptying in mice and ACh-induced or 5-HT-induced contraction in vitro gastric muscle in rats. Meanwhile, effect of anti-emetic extract on concentration-response curve to ACh, 5-HT, histamine was investigated. Result: 50% ethanol extract and 70% ethanol extract were identified as having significantly stronger anti-emetic activities with little side effect, which showed the significant effect on concentration-response curve to ACh, 5-HT, histamine. Conclusion: 50% ethanol extract and 70% ethanol extract contain more anti-emetic fractions, more anti-emetic fractions can be gained at the concentrations of 50% and 70% ethanol; the mechanism of anti-emetic effect is related to its antagonism to the receptors of ACh, 5-HT, histamine.

关 键 词：醇提 吴茱萸汤 镇吐药 呕吐 动物实验 

分 类 号：R289.5[医药卫生—方剂学]