咬合力丧失对大鼠牙周组织白细胞介素-1β表达的影响  

Effect of loss of bite force on interluekin-1βexpression in rat periodontium

作　　者：袁林[1] 周伟东[2] 

机构地区：[1]第一军医大学珠江医院口腔科,广东广州510282 [2]第一军医大学珠江医院肾内科,广东广州510282

出　　处：《第一军医大学学报》2002年第10期925-927,共3页Journal of First Military Medical University

摘　　要：:目的检测正常咬合力及咬合力丧失状态下,白细胞介素-1β(IL-1β)在大鼠牙周细胞中的动态变化,初步探讨IL-1β在牙周组织改建中的作用。方法选用Wistar大鼠建立正常咬合力及咬合力丧失动物模型。采用HE染色和免疫组化方法,观察两种咬合力状态下牙周形态的变化以及牙周组织中IL-1β表达的动态变化。结果咬合力丧失状态下,形态学显示牙周膜稀疏、结构紊乱,牙槽骨吸收;免疫组化显示咬合力丧失诱导IL-1β在牙周细胞中表达较正常咬合力时显著增强。结论牙周组织结构与功能在改建中具有一致性。Objective To study the expression of interluekin-1β(IL-1β) in the fibroblasts and osteoblasts in the periodontal ligament cells(PDLC) of normal rats and rats with loss of bite force, to explain the molecular mechanism of periodontium remodeling. Methods Rat models of lost bite force were established using Wistar rats. The changes in the histological morphology of the periodontium and the expression of IL-1βin the PDLC and osteoblasts were observed in both normal rats and the rat models using HE staining and immunohistochemistry techniques. Results In rat models of lost bite force, the periodontal ligament structure appeared to be disorganized and absorption occurred in the alveolar bone. The expression level of IL-1βin the PDLC and osteoblasts was significantly enhanced in comparison with that in the normal rats, as demonstrated by immunohistochemical study. Conclusion Loss of bite force may induce structural disorganization of the periodontal ligament and absorption in the alveolar bone, and the expression level of IL-1βin PDLC and osteoblasts show significant elevation, indicating that the changes in histological morphology is closely coordinated with the functional changes in the periodontium.

关 键 词：白细胞介素1 牙周组织 咬合力 疾病模型 动物 

分 类 号：R780.2[医药卫生—口腔医学]