二甲基苯蒽和苯并(a)芘对小鼠肝脏金属硫蛋白及氧化损伤的诱导作用  

Effect of 12-dimethylbenz(a)athrancene and benzo(a)pyrene on metallothionein expression and lipid peroxidation in mouse liver

作　　者：张宝旭[1] 阮明[1] 邱飞婵[1] 贾凤兰[1] 魏雪涛[1] 蒋建军[1] 尚兰琴[1] 

机构地区：[1]北京大学公共卫生学院环境医学研究所毒理学系,北京100083

出　　处：《环境科学学报》2002年第6期764-767,共4页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金资助课题 (No .39970 6 4 8)

摘　　要：观察两种致癌性多环芳烃化合物二甲基苯蒽 (DMBA)和苯并 (a)芘 (BaP)对小鼠肝脏脂质过氧化产物和金属硫蛋白含量的影响 .选用雄性C5 7BL OLA12 9小鼠 ,观察DMBA和BaP各 5 0mg kg一次腹腔注射染毒后 2 4、4 8、72和 14 4h后的动物肝脏重量、金属硫蛋白 (MT)含量和脂质过氧化程度的变化 .MT测定采用镉 血红蛋白亲和分析法 ;脂质过氧化指标为硫代巴比妥酸测定丙二醛法 .结果表明 ,DMBA和BaP均能引起小鼠肝脏重量增加、MT含量增加和MDA含量增加 .随着DMBA和BaP染毒后的时间延长小鼠肝脏的重量不断增加 ,呈现时间效应关系 .小鼠肝脏MT的含量在染毒后 2 4和 4 8h后明显增加 1倍 .肝脏MDA的含量增加在染毒后 2 4h后达到高峰 .本研究观察到DMBA和BaP可以诱导肝脏MT增加 .MT的增加与MDA的增加呈现一定平行的关系 .提示MT诱导可能与多环芳烃化合物所致的氧化损伤有关 .To study the induction of metallothionein (MT) and lipid peroxidation by two polycyclic aromatic hydrocarbons (PAHs), 7,12 dimethylbenz[a]athrancene (DMBA) and benzo[a]pyrene (BaP) in mice liver. Male mice were treated with DMBA or BaP i.p. at the dose of 50?mg/kg body weight for 0, 24, 48, 72 and 144?h, respectively. Liver MT was measured by the cadmium/hemoglobin saturation assay. Lipid peroxidation product (malondialdehyde, MDA) was measured as thiobarbituric acid reactive substances. After DMBA or BaP treatment, mice liver weight, MT and MDA increased in amount. Liver boby weight ratio increased significantly in a time dependent manner. MT was significantly induced vs control after 24?h with PAHs treatment. Liver MDA level was elevated one fold after 24?h treatment. Liver MT has been induced by PAHs. The correlation between MT expression and MDA level suggest that MT may be related with oxidative stress caused by PAHs.

关 键 词：肝脏 氧化损伤 金属硫蛋白 二甲基苯蒽 苯并(A)芘 脂质过氧化 致癌物质 环境污染物 毒性机理 多环芳烃 

分 类 号：R994.6[医药卫生—毒理学] X503.[医药卫生—药学]