大鼠甲状腺髓样癌细胞系CA-77在不同培养条件下的生物学特性  

Biological characteristics of rat medullary thyroid carcinoma cell line CA-77 in different culture conditions

作　　者：马宏敏[1] 王锦玲[1] 邱建华[1] 

机构地区：[1]第四军医大学西京医院全军耳鼻咽喉专科中心,陕西西安710033

出　　处：《第四军医大学学报》2002年第19期1754-1756,共3页Journal of the Fourth Military Medical University

基　　金：全军"九五"重点课题资助项目 (98B0 10 )

摘　　要：目的　观察大鼠甲状腺髓样癌细胞系 CA- 77在不同培养条件下生长、增殖、降钙素 (CT)及降钙素基因相关肽(CGRP)合成表达等情况 ,探讨 CA- 77细胞的培养条件 .方法　分别采用 DMEM,RPMI16 4 0和 DMEM/ Ham's F12 (1∶1)含血清培养液及无血清培养液进行 CA- 77细胞培养 ,分别绘制细胞生长曲线和 CT及 CGRP的免疫组化染色 .结果　CA- 77细胞在 DMEM,RPMI16 4 0和 DMEM/ Ham's F12(1∶ 1)含血清培养液和无血清培养液中均可保持其良好的生物特性 .结论　 CA- 77可以在常用培养液中培养 。AIM To investigate the culture conditions for rat medullary thyroid carcinoma cell line CA 77, the differences in growth, proliferation, calcitonin (CT) and calcitonin gene related peptide (CGRP) protein synthesis of the CA 77 cells incubated in varied culture conditions. METHODS CA 77 cells were cultivated in different culture systems, as DMEM, RPMI 1640, DMEM/Ham's F12 (1∶1) with 10% fetal bovine serum and serum free medium. The cell growth curves were drawn. CT and CGRP expression in the cellswere detected with immunohistochemistry. RESULTS The biological characteristics of CA 77 cells were maintained, whether incubated in DMEM, RPMI 1640, DMEM/Ham's F12 (1∶1) with 100 mL·L -1 fetal bovine serum or serum free medium. CONCLUSION CA 77 cell line could be cultured in common medium and its biological characteristics could be maintained stable in serum free medium.

关 键 词：甲状腺髓样癌 CA-77 细胞培养 大鼠 

分 类 号：R736[医药卫生—肿瘤]