低碘对亲、子代大鼠甲状腺形态结构及摄碘功能的影响  

Impact of low iodine on thyroid structure and iodine uptake function in parental and second filial generation rats

作　　者：陈洁 张玥 木叶色尔·艾尼瓦尔 王生玲[2] 张格祥[1] Chen Jie;Zhang Yue;Muyeseer·Ainiwaer;Wang Shengling;Zhang Gexiang(Institute of Nutrition and Food Hygiene,School of Public Health,Lanzhou University,Lanzhou 730000,China;Department for Endemic Disease Control and Prevention,Xinjiang Uygur A utonomous Regional Center for DiseaseControl and Prevention,Urumqi 830002,China)

机构地区：[1]兰州大学公共卫生学院营养与食品卫生学研究所,730000 [2]新疆维吾尔自治区疾病预防控制中心地方病防制科,乌鲁木齐830002

出　　处：《中华地方病学杂志》2019年第7期521-526,共6页Chinese Journal of Endemiology

基　　金：国家自然科学基金(81160334)

摘　　要：目的观察长期摄入低碘饲料对亲代和子二代大鼠甲状腺形态结构及钠碘转运体(NIS)的影响,并探索NIS蛋白表达量在碘缺乏病中的变化,探讨碘缺乏病的发生机制。方法参考《实验动物配合饲料营养成分》(GB 14924.3-2010),以新疆传统克汀病疫区广泛种植且食用率较高的农作物为主要饲料组分,配制不同低碘饲料(碘含量分别约为50、20 μg/kg);采用三代两窝法建立碘缺乏病大鼠模型,即选择断乳后的SPF级Wistar大鼠132只,雌雄各半,按体质量采用随机数字表法分为3组:对照组(N,52只,其中雌鼠22只,雄鼠30只,饲料中碘含量约为300 μg/kg),低碘1、2组(LⅠ、LⅡ,各40只,其中雌鼠22只,雄鼠18只)。各组大鼠饲喂至3、6、9个月各处死12只,雌雄各半,分离并进行甲状腺相对重量计算及大体结构、显微镜下病理学观察,蛋白免疫印迹(Western Blot)技术测定NIS蛋白表达量。亲代雌鼠在饲喂至3个月时各组选择4只雌鼠与N组雄鼠进行1∶1合笼交配,出生的子鼠按同样方法饲喂3个月再进行传代一次,子二代大鼠继续按母鼠组别进行饲喂,饲喂至3、6、9个月各处死10 - 12只大鼠,采集样本,观察指标同亲代大鼠。结果LⅠ、LⅡ组亲代大鼠甲状腺相对重量(mg/100 g体重)在低碘6、9个月均大于N组[雌性:(19.67 ± 5.60)、(23.81 ± 4.08)比(10.14 ± 1.20),(22.24 ± 2.06)、(33.51 ± 3.24)比(9.80 ± 1.96);雄性:(13.0 ± 3.70)、(13.84 ± 4.08)比(5.90 ± 1.20),(14.20 ± 2.67)、(19.98 ± 2.84)比(6.06 ± 0.76),P均< 0.05],且雌鼠在3、6、9个月均高于雄鼠(P均< 0.05);LⅠ、LⅡ组子二代甲状腺相对重量在低碘3、6、9个月时均大于N组(P均<0.05),除6个月的LⅠ组,其余各组雌鼠均高于雄鼠(P均< 0.05)。随着低碘进程,LⅠ、LⅡ组亲代和子二代大鼠甲状腺均出现颜色加深、充血和肿大现象。显微镜下病理学观察,各代大鼠甲状腺均呈现滤泡增多、滤泡腔变小、上皮细胞增生现象,�ObjectiveTo observe the effect of long-term intake of low iodine diet on thyroid morphological structure and sodium iodine transporter (NIS) in parental and second filial generation rats, and to explore the changes of NIS protein expression in iodine deficiency disease(IDD), so as to further verify and explore the mechanism of IDD.MethodsReferring to "Nutritional Composition of Experimental Animals With Feed" (GB 14924.3-2010), the crops widely planted and with high edible rate in the traditional cretinism epidemic area of Xinjiang were used as the main feed components to prepare different levels of iodine (low iodine groups 1 and 2 feed the iodine contents were about 50 and 20 μg/kg). The IDD rat model was established by the three-generation two-nest method, that is, 132 SPF Wistar rats were selected after weaning, half males and half females, randomly divided into 3 groups according to body mass by random number table method: control group (N, 52 rats, 22 females, 30 males, the iodine content was about 300 μg/kg), low iodine groups 1 and 2 (LⅠ, LⅡ, 40 rats, 22 females, 18 males in each group). Twelve rats in each group were sacrificed at the end of 3, 6, 9 months, respectively, half males and half females. The relative weight of thyroid was calculated and the gross structure and microscopic pathology were observed. The expression of NIS protein was determined by Western blotting. At the end of 3 months after feeding, four female rats in each group were selected to mate with male rats in group N in 1 ∶ 1, and the newborn mice were fed the same way for three months and then subcultured again. The rat generation continued to be fed according to the mother group, and 10 - 12 rats were sacrificed at the end of 3, 6, 9 months, respectively, and the specimens and observation indexes results were collected same as the parental rats.ResultsThe relative weight of the thyroid in parental LⅠ and LⅡ groups were higher than those in the N group at 6 and 9 months [female: (19.67 ± 5.60), (23.81 ± 4.08) vs (10.14

关 键 词：碘 大鼠 甲状腺 钠碘转运体 

分 类 号：R73[医药卫生—肿瘤]