胰腺导管腺癌中microRNA-21与TGF-β1信号通路相关  

MicroRNA-21 correlates TGF-β1 pathway of pancreatic ductal adenocarcinoma

作　　者：吴翔[1] 卓书伟 郑才玲 高戈 WU Xiang;ZHUO Shuwei;ZHENG Cailing;GAO Ge(Clinical Laboratory,Hainan Provincial Hospital of Chinese Medicine,Haikou 570203;Department of Laboratory Medicine,Third Xiangya Hospital,Central South University,Changsha 410013,China)

机构地区：[1]海南省中医院检验科,海口570203 [2]中南大学湘雅三医院检验科,长沙410013

出　　处：《中南大学学报（医学版）》2019年第7期749-756,共8页Journal of Central South University ：Medical Science

基　　金：海南省自然科学基金(818MS159)~~

摘　　要：目的:对胰腺导管腺癌样本进行基因分析,筛选与胰腺导管腺癌相关的microRNA(miRNA),并初步分析目标miRNA与胰腺癌转化生长因子-β1(TGF-β1)信号通路的相关性。方法:收集在海南省中医院住院的胰腺导管癌患者术前外周静脉血血清标本19份,健康体检人群的外周静脉血血清标本21份作为非胰腺导管癌对照组。利用GCBI(Gene-Cloud Biotechnology Information)数据平台筛选与胰腺导管腺癌样本有关的基因并对其进行生物信息学分析,将筛选出的基因作为研究对象,用real-time PCR和蛋白质印迹法验证该基因在胰腺导管腺癌中的表达。通过改变胰腺导管腺癌细胞中该基因的表达水平观察其与TGF-β1之间的关系。结果:通过聚类分析和基因功能富集分析筛选出miRNA-21为胰腺导管癌相关基因。MiRNA-21在胰腺导管腺癌患者体内高表达。在miRNA-21过表达的PANC-1细胞中,TGF-β1表达受到抑制;但当miRNA-21的表达受到抑制时,TGF-β1的表达明显上升。结论:MiRNA-21在胰腺导管腺癌患者体内高表达,可调控TGF-β1的表达,从而参与胰腺导管腺癌的发生发展。Objective:To conduct genetic analysis of pancreatic ductal adenocarcinoma tissues and analyze the correlation between targeted microRNA(miRNA)and pathways in pancreatic ductal adenocarcinoma.Methods:We collected 19 samples of peripheral venous blood serum from patients with pancreatic ductal adenocarcinoma in Hainan Provincial Hospital of Chinese Medicine,and also collected 21 blood serum samples as a control group of non-pancreatic ductal adenocarcinoma.We used the bioinformatics analysis of literature GCBI data platform for screening and analyzing the genetics of pancreatic ductal adenocarcinoma samples.Through GCBI data platform of hierarchy clustering analysis and the enrichment of gene function analysis,the relevant miRNA was screened as a research object in patients with pancreatic ductal adenocarcinoma.The miRNA was screened by literature analysis and pancreatic cancer gene analysis.Real-time PCR and Western blotting were carried out to study the relationship between the selected miRNA and TGF-β1 by overexpression and suppression of the gene in pancreatic ductal adenocarcinoma cells.Results:MiRNA-21 was screened as a gene associated with pancreatic ductal carcinoma via hierarchy clustering analysis and gene function analysis.MiRNA-21 was highly expressed in the pancreatic ductal carcinoma patients.Expressions of TGF-β1 were inhibired in miRNA-21 overexpressed PANC-1.While the expression of miRNA-21 was inhibited,TGF-β1 expression increased obviously.Conclusion:MiRNA-21 is highly expressed in patients with pancreatic ductal adenocarcinoma,can regulate the expression of TGF-β1,which may be a mechanism of miRNA-21 in pancreatic ductal adenocarcinoma.

关 键 词：胰腺导管腺癌 MICRORNA-21 转化生长因子-Β1 

分 类 号：R73[医药卫生—肿瘤]