小麦TaLEA3-1基因的克隆、表达与渗透胁迫功能分析  

Cloning, Expression and Osmotic-stress Function Analysis of TaLEA3-1 Gene in Wheat

作　　者：王莲哲 王渊 柳超奇 李冰冰 Wang Lianzhe;Wang Yuan;Liu Chaoqi;Li Bingbing(College of Life Science and Engineering,Henan University of Urban Construction,Pingdingshan467036)

机构地区：[1]河南城建学院生命科学与工程学院

出　　处：《分子植物育种》2019年第14期4524-4528,共5页Molecular Plant Breeding

基　　金：河南省科技攻关项目(182102110476);平顶山市科技攻关项目(2017007(7.3))共同资助

摘　　要：胚胎发育晚期丰富蛋白(late embryogenesis abundant,LEA)受逆境胁迫诱导,是一种逆境表达蛋白,参与植物对逆境胁迫的响应。本研究从小麦cDNA文库中克隆得到一个新的小麦TaLEA3-1基因,该基因全长696bp,编码231个蛋白,属于LEA3亚组。该基因在小麦根、茎、叶、穗和种子中均有表达,其中种子和叶片的相对表达量高。不同逆境胁迫处理下qRT-PCR结果显示,TaLEA3-1广泛响应PEG、NaCl、低温和脱落酸(ABA)处理,表达量显著上调。将该基因转化酵母INVSC1株系并诱导外源基因表达,转基因酵母在渗透胁迫处理下相比对照组有更好的生长状态。研究结果表明小麦TaLEA3-1基因具有抵御渗透胁迫的功能,为进一步深入的功能机制研究提供基础数据与基因资源。Late embryogenesis abundant(LEA)is a kind of stress-expressing protein which is induced by stress,and plays an important role in plant responding to abiotic stress.In this study,from wheat cDNA library,we cloned a new LEA gene of wheat.The gene was 696 bp in length and encoded 231 proteins,belonging to the LEA3 subgroup.The gene was expressed in wheat roots,stems,leaves,spikes and seeds,and had higher relative expression in seeds and leaves.The results of qRT-PCR under different stress showed that TaLEA3-1 was widely responded to PEG,cold,NaCl and ABA treatments,and its expression was significantly increased.The gene was transformed into yeast INVSC1 strain and the expression of exogenous genes was induced.The transgenic yeast INVSC1 had better growth than the control under osmosis stress.The results showed that the wheat TaLEA3-1 gene might have the function of resisting osmotic stress,and would provide basic data and genetic resources for further functional mechanism research.

关 键 词：小麦 LEA蛋白 基因表达 渗透胁迫 酵母 

分 类 号：S51[农业科学—作物学]