荜茇酰胺对KRAS突变肺腺癌细胞增殖和凋亡的影响研究  

Effect of piperlongumine on proliferation and apoptosis of KRAS-mutant lung adenocarcinoma cells

作　　者：叶武 钦光跃[1] 唐婷玉[1] 杜坚宗[1] 刘娟 黎雪芳 YE Wu;QIN Guangyue;TANG Tingyu(Department of Respiratory Medicine,Zhejiang Hospital,Hangzhou 310013,China)

机构地区：[1]浙江医院呼吸内科,杭州310013 [2]浙江医院心内科,杭州310013

出　　处：《浙江医学》2019年第15期1584-1586,1590,共4页Zhejiang Medical Journal

摘　　要：目的探讨荜茇酰胺对Kirsten鼠肉瘤病毒癌基因(KRAS)突变肺腺癌细胞增殖和凋亡的影响。方法使用不同浓度梯度的荜茇酰胺干预KRAS突变肺腺癌细胞A549,然后采用CCK-8法检测A549细胞活力,Annexin V-FITC和PI双染色法检测细胞凋亡率,Western blot法检测细胞凋亡相关蛋白PARP、BCL2和自噬相关蛋白LC3B的表达水平。结果荜茇酰胺干预A549细胞后,细胞活力降低,细胞凋亡率升高,且荜茇酰胺浓度越高,细胞活力越低,细胞凋亡率越高。荜茇酰胺可上调细胞PARP、LC3BⅡ表达水平,下调BCL2表达水平。结论荜茇酰胺可抑制KRAS突变肺腺癌细胞增殖,诱导细胞凋亡,或可成为治疗KRAS突变肺腺癌的新靶向药物。Objective To investigate the effects of piperlongumine on proliferation and apoptosis of lung adenocarcinoma cells with Kirsten murine sarcoma virus oncogene(KRAS)mutation.Methods KRAS mutant lung adenocarcinoma A549 cells were treated with different concentrations of piperlongumine.Cell viability was determined by CCK-8 assay,and cell apoptosis was detected by Annexin V/PI double staining test.Western blotting was used to detect the expression of apoptosis-related proteins PARP,BCL2 and autophagy-related protein LC3B.Results After treatment with piperlongumine,the viability of A549 cells decreased and the apoptosis rate increased.The higher concentration of piperlongumine resulted in the lower cell viability and the higher apoptotic rate.Treatment with piperlongumine increased the expression of PARP cleavage fragment and LC3BⅡ,and downregulated the expression of BCL2.Conclusion Piperlongumine inhibits the proliferation of KRAS-mutant lung adenocarcinoma cells and induces apoptosis,indicating that it might be used as a targeted drug for the treatment of KRAS-mutant lung adenocarcinoma.

关 键 词：荜茇酰胺 KRAS突变 肺腺癌 凋亡 自噬 

分 类 号：R73[医药卫生—肿瘤]