微小RNA在双生子单冠心病患者中的差异表达  

Differential expression and regulation of microRNAs in patients with coronary heart disease

作　　者：秦聪 杨飞[2] Qin Cong;Yang Fei(Cardiovascular Surgery,the First Affiliated Hospital of Air Force Medical University of PLA,Xi’an 710032,China;Department of Rehabilitation,Xi’an Chinese Medicine Encephalopathy Hospital,Shaanxi University of Traditional Chinese Medicine,Xi’an 710032,China)

机构地区：[1]中国人民解放军空军军医大学第一附属医院(西京医院)心血管外科,西安710032 [2]陕西中医药大学附属西安中医脑病医院康复医学科,710032

出　　处：《中国心血管杂志》2019年第4期346-351,共6页Chinese Journal of Cardiovascular Medicine

摘　　要：目的引用双生子经典研究途径,筛选冠心病患者外周血差异表达的微小RNA(miR),分析miR在冠心病患者中的调控作用。方法选择两对冠心病–健康双生子为研究对象,其中2例冠心病患者为研究组,2名健康者为对照组,分别抽取外周血5 ml,TRIzol法提取细胞总RNA,miRCURYTM LNA Array杂交芯片筛选差异表达miR。采用MirBase、TargetScan和MIRDB软件预测差异性miR的靶基因;DAVID软件进行靶基因GO分析和KEGG分析,Cytoscape软件绘制基因调控网络图。结果共筛选出43条差异表达miRs,其中12条表达上调,31条表达下调。最为显著的hsa-miR-1066-5p、hsa-miR-3074-3p、hsa-miR-4505、hsa-miR-3658和hsa-miR-4459分别下调10倍以上,hsa-miR-4699-3p上调8倍以上,靶基因GO注释和KEGG分析显示下调的miRs参与了大量免疫因子调控。结论冠心病患者差异表达的miRs对免疫因子的调控,可能是导致冠心病发病的关键作用之一。Objective Introducing the research approach of twins classics,to screen differentially expressed microRNAs(miRs)in peripheral blood of patients with coronary heart disease and analyze the regulatory mechanism of miR in patients with coronary heart disease.Methods Two pairs of coronary heart disease-healthy twins were selected as the study subjects.Two patients with coronary heart disease were the study group,and two healthy subjects were the control group.5 ml peripheral blood was taken,and total RNA was extracted by TRIzol method.miRCURYTM LNA Array Hybridization chips screen differentially express miR.MirBase,TargetScan and MIRDB software were used to predict differential miR target genes;DAVID software was used for target gene GO analysis and KEGG analysis,and Cytoscape software was used to map gene regulatory networks.Results A total of 43 differentially expressed miRs were screened,of which 12 were up-regulated and 31 were down-regulated.Among them,the most significant hsa-miR-1066-5p,hsa-miR-3074-3p,hsa-miR-4505,hsa-miR-3658 and hsa-miR-4459 were down-regulated by 10 times or more,hsa-miR-4699-3p up-regulated by more than 8 fold,target gene GO annotation and KEGG analysis showed that down-regulated miRs were involved in the regulation of a large number of immune factors.Conclusions The differential regulation of immune factors by differentially expressed miRs in patients with coronary heart disease may be one of the key mechanisms for the pathogenesis of coronary heart disease.

关 键 词：冠状动脉疾病 微小RNA 靶基因 调控网络 

分 类 号：R73[医药卫生—肿瘤]