优化ATP荧光法检测医院次氯酸钠消毒后物体表面菌落数的稳定性观察  

Application of optimized ATP fluorescence method in NaClO disinfection

作　　者：张博 刘阳 樊海鑫 陈玉 赵小梨 张丽娟 陆红玲 ZHANG Bo;LIU Yang;FAN Haixin;CHEN Yu;ZHAO Xiaoli;ZHANG Lijuan;LU Hongling(Basic Medical College,Zunyi Medical University School,Zunyi,Guizhou 563000,China)

机构地区：[1]遵义医科大学基础医学院,贵州遵义563000

出　　处：《安徽医药》2019年第9期1830-1833,共4页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金（31860315）

摘　　要：目的观察优化ATP荧光法检测医院次氯酸钠消毒后物体表面菌落数的稳定性,拓展ATP荧光法在医院的应用范围。方法优化ATP荧光法检测试剂的成分,采用优化后的ATP荧光法对医院一种常用消毒药次氯酸钠(NaClO)消毒后的菌落数进行检测,并考察其检测结果与国标平皿法菌落数检测结果的相关性。结果优化后的ATP荧光法检测医院次氯酸钠消毒后的物体表面菌落总数与国标平皿法菌落总数检测结果有很好的相关性,相关系数为0.985,Y=0.062 7X+12.453(R2=0.962 5),并在此基础上制定了荧光法检测的半定量标准。结论ATP荧光法可以应用于医院次氯酸钠消毒后物表的检测。Objective This paper aims to by observing the number of colony after ATP fluorescence detectionto optimize andex?pand the application scope of ATP fluorescence method in hospitals.Methods The composition of ATP fluorescence detection re?agent was optimized,and then NaClO,a common disinfection component,was selected for determination.Results It was found that the optimized ATP fluorescence method whichused to detecthospital NaClO disinfection surface had a good correlation with the na?tional standard plate method colony count detection results,Y=0.062 7X+12.453(R2=0.962 5),and on this basis,a semi?quantita?tive standard for fluorescence detection was established.Conclusion ATP fluorescence detection results have significant correla?tion with the national standard colony count detection method,with a correlation coefficient of 0.985.This method can be applied to the detection of the surface which disinfected after NaClO in hospitals.

关 键 词：消毒检测 次氯酸钠 ATP荧光法 菌落总数 

分 类 号：S85[农业科学—兽医学]