一株高适应性Nitrosomonas eutropha CZ-4的脱氨特性  

Nitrogen removal characteristics of a highly adaptable Nitrosomonas eutropha CZ-4

作　　者：熊英[1] 向斯[1,2] 程凯 XIONG Ying;XIANG Si;CHENG Kai(Hubei Key Laboratory of Ecological Restoration for River-Lakes and Algal Utilization for College of Resources and Environmental Engineering,Hubei University of Technology,Wuhan 430068,China;Wuhan Micro-Glory Environmental Technology Innovation Co.Ltd,Wuhan 430068,China)

机构地区：[1]湖北工业大学资源与环境工程学院,河湖生态修复与藻类利用湖北省重点实验室,湖北武汉430068 [2]武汉微盛科创环境生物科技有限公司,湖北武汉430068

出　　处：《中国环境科学》2019年第8期3365-3372,共8页China Environmental Science

基　　金：国家科技重大专项(2017ZX07602002)

摘　　要：从垃圾渗滤液中分离得到了一株亚硝化单胞菌Nitrosomonas eutropha CZ-4,其16S rDNA序列与N.eutropha C91的相似性达99%.研究了pH值、温度、游离亚硝酸浓度、盐度等对其生长的影响,并测试了其在垃圾渗滤液、黑臭水和富营养化湖水中的脱氨效果.结果表明,该菌的最适生长pH值为7.3~8.7,最适生长温度为30.9℃,游离亚硝酸和盐度对该菌的半数抑制浓度分别约为0.11mg/L与2%.在最佳发酵条件下,该菌的最大氨氮去除速率为58mg/(L?h),最短倍增时间为8.2h;在不同类型的污水/地表水(初始氨氮浓度为0.66~603mg/L)中,该菌的最大氨氮去除速率为11.4mg/(L?h),最短倍增时间为10.9h,最低残余氨氮浓度为0.11mg/L.A Nitrosomonas strain was isolated from the landfill leachate.It was named as Nitrosomonas eutropha CZ-4 for its 16s rDNA sequence was highly similar to N.eutropha C91at an identity of 99%.This work studied the influences of pH,temperature,free nitrous acid concentration and salinity on the growth of N.eutropha CZ-4,as well as its NH3-N removal abilities in landfill leachate,black odor water and eutrophic lake water.The results showed that the optimum growth pH of the strain was 7.3~8.7,the optimum growth temperature was 30.9°C,and the IC50 of free nitrous acid and salinity was about 0.11mg/L and 2%(in terms of NaCl),respectively.Under the optimal fermentation condition,the maximum NH3-N removal rate reached 58mg/(L?h),and the shortest doubling time was 8.2h.In different types of sewage/surface water(their initial ammonia concentration ranged from 0.66mg/L to 603mg/L),the NH3-N removal rate was up to 11.4mg/(L?h),the doubling time was as short as 10.9h,and the residual NH3-N concentration was down to 0.11mg/L.

关 键 词：自养氨氧化菌 垃圾渗滤液 亚硝化单胞菌 氨氮去除速率 倍增时间 

分 类 号：X172[环境科学与工程—环境科学] X703.1