不同前处理方法对测定动物饲料中多种霉菌毒素的影响  

Effect of different pretreatment methods on the determination of multiple mycotoxins in animal feed

作　　者：邵玉芳[1] 邵世勤[1] SHAO Yu-fang;SHAO Shi-qin

机构地区：[1]内蒙古农业大学

出　　处：《饲料研究》2019年第7期67-71,共5页Feed Research

基　　金：大型仪器开放共享检验检测及升级改造“微波消解火焰原子吸收法测定饲料中多种元素(项目编号:内财科[2018]1377)”

摘　　要：通过稀释液和免疫亲和柱这2种不同的前处理方法,基于LC-MS/MS分析技术同时测定动物饲料中的8种霉菌毒素。通过3种不同浓度水平的一系列加标饲料样品验证不同净化方式对检测结果的影响。结果表明:免疫亲和柱法的信噪比较高,其回收率为75.3%~121.0%。稀释法的回收率为89.0%~110.0%,此方法精确度更高,重现性CV值分别为稀释法8.7%~23.4%,免疫亲和柱法13.1%~37.5%。这2种方法均符合霉菌毒素分析的要求,可有效地应用于多种分析物质的测定。尽管稀释法具有较好的精密度和重现性,但免疫亲和净化法灵敏度较高,且具有较低的基质效应和检测限,这在饲料中霉菌毒素的多重检测中具有较大优势。The aim of the study was performance comparison of two clean-up procedures(dilutions versus immunoaffinity columns) in the simultaneous determination of eight mycotoxins in the animal feed with the LC-MS/MS technique. Both of the procedures were validated by analysis on a series of spiked feed samples(n=6) at three different concentration levels. Better signal to noise ratios were observed for immunoaffinity clean-up. The recoveries of analyses were in the range 88.0%~110.0% for the dilution procedure and 78.0%~120.0% for the immunoaffinity clean-up. The dilution procedure was more precise(CV of the within-laboratory reproducibility for it was 7.8%~22.4% in comparison to 12.0%~35.5% for the immunoaffinity cleanup. The results show that both procedures fulfilled the requirements for mycotoxin analysis and can be used successfully in multi-analyte determination. Although the dilution procedure shows better precision and trueness, the immunoaffinity clean-up procedure has advantages in more complex feed samples thanks to lower matrix effect and limits of detections.

关 键 词：动物饲料 免疫亲和柱 稀释法 霉菌毒素 LC-MS/MS法 

分 类 号：Q93[生物学—微生物学]