嗜酸乳杆菌对斜带石斑鱼幼鱼生长、消化性能和抗病力的影响  

EFFECTS OF LACTOBACILLUS ACIDOPHILUS (GIM:1.730) ON GROWTH,DIGESTIVE CAPACITY, DISEASE RESISTANCE OF JUVENILE, EPINEPHELUS COIOIDES

作　　者：李军亮 杨奇慧 谭北平 董晓慧 迟淑艳 刘泓宇 章双 张海涛 LI Jun-Liang;YANG Qi-Hui;TAN Bei-Ping;DONG Xiao-Hui;CHI Shu-Yan;LIU Hong-Yu;ZHANG Shuang;ZHANG Hai-Tao(Aquatic Animal Nutrition and Feed Laboratory,Fisheries College,Guangdong Ocean University,Zhanjiang,524088,China;Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture,Zhanjiang 524074,China)

机构地区：[1]广东海洋大学水产学院,水产动物营养与饲料实验室,湛江524088 [2]农业部南方水产与畜禽饲料重点实验室,湛江524074

出　　处：《水生生物学报》2019年第5期992-1000,共9页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(31772864和31802316);现代农业产业技术体系专项资金(CARS-47);广东省科技厅社会发展领域科技计划项目(2013B021100017);广东省自然科学基金面上项目(2015A030313621和2016A030313749);广东省创新强校工程项目(2015050206)资助~~

摘　　要：研究嗜酸乳杆菌(Lactobacillus acidophilus,GIM:1.730)对斜带石斑鱼(Epinephelus coioides)幼鱼生长、消化酶活性、抗病力和相关酶mRNA表达的影响。选取720尾初始均重为(5.60±0.05)g的斜带石斑鱼幼鱼为研究对象,共设8个处理,每个处理3个重复。投喂不同添加比例(0、0.1%、0.2%、0.4%、0.6%、0.8%、1.0%和1.2%)嗜酸乳杆菌的饲料,养殖期8周。结果表明:增重率(Weight gain rate,WGR)与特定生长率(Specific growth rate,SGR)实验组均显著高于对照组(P<0.05)。当嗜酸乳杆菌添加0.6%时,饲料系数(Feed conversion ratio,FCR)最低,其他各组显著低于对照组(P<0.05),而蛋白质效率(Protein efficiency ratio,PER)变化规律则与FCR相反。肝脏和肠蛋白酶(Trypsin,TRY)、淀粉酶(Amylase,AMY)活性实验组均显著高于对照组(P<0.05)。肝脏TRY、AMY mRNA表达,实验组均显著高于对照组(P<0.05)。其中,肝脏淀粉酶、蛋白酶活性在添加量为0.6%达到最大值,肠蛋白酶、淀粉酶活性分别在添加量0.2%和0.4%达到最大值。以哈维弧菌(Vibrio harveyi)攻毒7d,石斑鱼攻毒后成活率(Survival rate,SR)随着嗜酸乳杆菌添加量的增加显著升高(P<0.05)。以WGR为判断依据得出,在饲料中添加0.42%嗜酸乳杆菌(0.72×10^8 cfu/g)可显著促进斜带石斑鱼幼鱼的生长,并提高消化酶活性和抗病力。The effects of dietary Lactobacillus acidophilus on growth performance,digestive enzyme activities,premunition and related enzyme gene mRNA expression of Epinephelus coiodes were assessed in the current study.Seven hundred and twenty healthy fish with an average body weight of(5.60±0.05)g were randomly divided into 8 groups with 3 replicates per group and 30 fish per replicate.The fish were fed a basal diet and an experimental diet that were supplemented with L.acidophilus at the percentage of 0,0.1,0.2,0.4,0.6,0.8,1.0 and 1.2%for 8 weeks,respectively.The WGR and SGR increased first and then decreased with increased L.acidophilus,but they were significantly higher than those of the control group(P<0.05).The FCR reached the lowest value in 0.6%group that was significantly lower than other group(P<0.05).However,the PER level showed an opposite trend to the FCR.The activities of TRY and AMY in the liver and intestine tended to increase first and decrease thereafter.The liver TRY and AMY mRNA level of the experimental group were significantly higher than those in the control group(P<0.05).Among them,liver amylase and protease activity reached the maximum level at 0.6%,and intestinal protease and amylase activities reached the maximum level at 0.2%and 0.4%,respectively.The fish survival rate significantly increased with the increased Vibrio harvey for 7 days(P<0.05).These results reveal that 0.42%Lactobacillus acidophilus(0.72×10^8 cfu/mL)in the feed could significantly promote the growth and increase the digestive enzyme activity and disease resistance for juvenile grouper.

关 键 词：斜带石斑鱼 嗜酸乳杆菌 生长 消化性能 抗病力 

分 类 号：S965.3[农业科学—水产养殖]