细胞周期分析新方法  

New Methods for Cell Cycle Analysis

作　　者：方涵书 郎明非[1] 孙晶[2] FANG Han-Shu;LANG Ming-Fei;SUN Jing(Medical College,Dalian University,Dalian 116622,China;College of Environmental and Chemical Engineering,Dalian University,Dalian 116622,China)

机构地区：[1]大连大学医学院,大连116622 [2]大连大学环境与化学工程学院,大连116622

出　　处：《分析化学》2019年第9期1293-1301,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21505013);大连市科技创新基金项目(No.2018J13SN087)资助~~

摘　　要：细胞周期是细胞生命活动的基本过程,对细胞周期的分析能更深入地了解疾病发生的本质。目前,分析细胞周期的方法以流式细胞术为主,主要是通过检测细胞内DNA含量和细胞周期特异性蛋白而分析细胞周期。随着分析技术的发展,细胞周期分析在图像分析、单细胞分析、减少样本量、提高通量等方面不断地提高和改进,近年出现了许多分析细胞周期的新方法,如成像流式细胞术、质谱流式细胞术、微流控技术、拉曼光谱法等,这些方法极大地丰富了细胞周期的检测方法,提高了细胞周期测量的精确度。本文对细胞周期的新分析方法进行了介绍,并对其发展方向进行了展望。Cell cycle is a basic process during the cell life.Analyzing cell cycles can better understand the nature of diseases.Currently,cell cycles are mainly analyzed by flow cytometry,which investigate cell cycles by measuring intracellular DNA contents and cell cycle specific proteins.With the development of technology,several new methods have emerged for cell cycle analysis in recent years to improve and enhance image analysis,single-cell analysis,sample reduction,and high throughput.These new methods including image flow cytometry,mass flow cytometry,microfluidic technology and Raman spectroscopy greatly expand the methods for cell cycle analysis and increase the accuracy of cell cycle measurements.Here we briefly introduced these new methods for cell cycle analysis.

关 键 词：细胞周期 细胞周期蛋白 DNA含量 微流控技术 流式细胞术 评述 

分 类 号：R73[医药卫生—肿瘤]