白细胞分化抗原基因多态性与缺血性脑卒中发生与发展的关系  

Relationship between Polymorphism of Leucocyte Differentiation Antigen Gene and Occurrence and Development of Ischemic Stroke

作　　者：闫安[1] 李春颖[2] Yan An;Li Chunying(Affiliated Hospital of Beihua University,Jilin 132011,China;Medical College of Beihua University,Jilin 132013,China)

机构地区：[1]北华大学附属医院,吉林吉林132011 [2]北华大学医学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2019年第5期659-662,共4页Journal of Beihua University（Natural Science）

基　　金：吉林市医疗卫生指导性科技计划项目(201737134);吉林省卫生与健康青年科技骨干培养计划项目(2018Q037)

摘　　要：目的探讨白细胞分化抗原基因多态性与缺血性脑卒中发生与发展的关系.方法选取缺血性脑卒中患者404例为观察组,同期体检的同年龄段健康人群398名为对照组.采集空腹外周静脉血,单碱基延伸PCR技术对白细胞分化抗原40基因rs1535045 C/T,rs3765459 C/T,rs4810485 C/T,rsl3040307G/A位点多态性进行分型.结果白细胞分化抗原40基因各位点分布情况显示:rs1535045 C/T位点中CC,CT,TT基因型之间差异具有统计学意义(P<0.01);rs3765459 C/T,rs4810485 C/T,rsl3040307 G/A位点各基因型之间差异无统计学意义(P>0.0 5).rs1535045 C/T位点等位基因频率在观察组与对照组间差异具有统计学意义(P<0.01);携带T突变型等位基因的缺血性脑卒中发生风险较携带C野生型等位基因明显增加,是携带C野生型的1.846倍.rs3765459 C/T,rs4810485 C/T,rsl3040307 G/A位点等位基因频率在观察组与对照组间差异无统计学意义(P>0.0 5).联合基因型分析显示:观察组携带TCCA,TCCG基因单倍型的比例明显高于对照组,携带CCCG基因单倍型的比例明显低于对照组,差异具有统计学意义(P<0.05);携带TCCA,TCCG基因单倍型明显增加缺血性脑卒中的发生风险,携带CCCG基因单倍型明显降低缺血性脑卒中的发生风险.结论白细胞分化抗原40基因rs1535045 C/T位点及TCCA,TCCG,CCCG单倍型与缺血性脑卒中的发生与发展可能存在相关性,其中携带T突变型等位基因可能为缺血性脑卒中的遗传易感基因.Objective To investigate the relationship between the polymorphism of leucocyte differentiation antigen gene and the occurrence and development of ischemic stroke.Method 404 patients with ischemic stroke were selected as the observation group,and 398 healthy people of the same age were selected as the control group during the same period of physical examination.The polymorphisms of rs1535045 C/T,rs3765459 C/T,rs4810485 C/T and rsl3040307 G/A locus of leucocyte differentiation antigen 40 gene were typed by fasting peripheral venous blood and single base extension PCR.Results The distribution of leucocyte differentiation antigen 40 gene at different sites showed that there were significant differences among CC,CT and TT genotypes at rs1535045 C/T locus(P<0.01);there was no significant difference among genotypes at rs3765459 C/T,rs4810485 C/T and rsl3040307 G/A locus(P>0.05).The frequency of C/T allele at rs1535045 in the observation group was significantly higher than that in the control group(P<0.01);the risk of ischemic stroke with T mutant allele was 1.846 times higher than that with C wild allele.There was no significant difference in allele frequencies of rs3765459 C/T,rs4810485 C/T and rsl3040307 G/A loci between the observation group and the control group(P>0.05).Combined genotype analysis showed that the proportion of TCCA and TCCG haplotypes in the observation group was significantly higher than that in the control group,and the proportion of CCCG haplotypes in the observation group was significantly lower than that in the control group(P<0.05).TCCA and TCCG haplotypes significantly increased the risk of ischemic stroke,and CCCG gene haplotypes were carried.Haploid significantly reduces the risk of ischemic stroke.Conclusion The rs1535045 C/T locus of leucocyte differentiation antigen 40 gene and haplotypes of TCCA,TCCG and CCCG may be related to the occurrence and development of ischemic stroke,among which T mutant allele may be a genetic susceptibility gene for ischemic stroke.

关 键 词：缺血性脑卒中 白细胞分化抗原40 基因多态性 

分 类 号：R743.3[医药卫生—神经病学与精神病学]