靶向细胞表面DcR3适配体促进肺鳞癌细胞凋亡的研究  

Study of targeted cell surface DcR3 adapter on promoting apoptosis of lung squamous cell carcinoma

作　　者：何大保 聂群 李宁 刘晓洪 张勇刚 HE Da-bao;NIE Qun;LI Ning;LIU Xiao-hong;ZHANG Yong-gang(Medical Laboratory Department,Longhua District Central Hospital,Shenzhen,Guangdong 518110,China;Laboratory Department,Maternal and Child Health Cure Hospital of Dapeng New District,Shenzhen,Guangdong 518120,China;Department of Oncology,Longhua District Central Hospital,Shenzhen,Guangdong 518110,China)

机构地区：[1]深圳市龙华区中心医院医学检验科,广东深圳518110 [2]深圳市大鹏新区妇幼保健院检验科,广东深圳518120 [3]深圳市龙华区中心医院肿瘤科,广东深圳518110

出　　处：《临床肺科杂志》2019年第9期1692-1696,共5页Journal of Clinical Pulmonary Medicine

基　　金：深圳市龙华区科技创新专项资金项目(No 2017081)

摘　　要：目的探讨靶向细胞表面DcR3适配体对肺鳞癌细胞SK-MES-1、NCI-H520增殖凋亡的影响。方法采用聚合酶链反应、凝胶电泳对SK-MES-1、NCI-H520细胞DcR3 mRNA表达水平进行检测,应用MTT法检测靶向细胞表面DcR3适配体对细胞增殖抑制率,流式细胞仪分析细胞周期分布,采用Western blot法分析FAS的表达变化。结果SK-MES-1细胞DcR3 mRNA相对表达水平为(1.134±0.115),靶向细胞表面DcR3适配体与细胞共孵育后DcR3 mRNA相对表达水平为(0.497±0.062),差异具有统计学意义(t=7.026,P<0.001);NCI-H520细胞DcR3 mRNA相对表达水平为(1.036±0.078),靶向细胞表面DcR3适配体与细胞共孵育后DcR3 mRNA相对表达水平为(0.419±0.028),差异具有统计学意义(t=7.193,P<0.001)。经靶向细胞表面DcR3适配体处理的G 0/G 1、S期SK-MES-1、NCI-H520细胞与未经处理的空白对照组相比,差异均具有统计学意义(P<0.05),其中G 0/G 1期细胞显著高于未经处理的空白对照组(P<0.05),S期细胞显著低于未经处理的空白对照组(P<0.05)。SK-MES-1、NCI-H520细胞经靶向细胞表面DcR3适配体处理后FAS蛋白表达量降低,且随着靶向细胞表面DcR3适配体浓度的加大而减低,差异具有统计学意义(F分别为8.024、12.419,<0.001)。结论DcR3可能参与肺鳞癌细胞的生长、增殖,为其治疗提供了新的靶点。Objective To investigate the effect of DcR3 adapter on targeted cell surface on proliferation and apoptosis of lung squamous cell carcinoma SK-MES-1 and NCI-H520 cells.Methods Polymerase chain reaction and gel electrophoresis were used to detect DcR3 mRNA in SK-MES-1 and NCI-H520 cells.The inhibition rate of DcR3 adapter on the target cell surface was detected by MTT and flow cytometry,and cell cycle distribution and changes of FAS expression were analyzed by Western blot method.Results The relative expression level of DcR3 in SK-MES-1 cells was(1.134±0.115).The relative expression level of DcR3 mRNA after co-incubation of DcR3 adapters on targeted cell surface was(0.497±0.062)(t=7.193,P<0.001).The relative expression level of DcR3 mRNA in NCI-H520 cells was(1.036±0.078).The relative expression level of DcR3 mRNA after co-incubation of DcR3 adapters on the target cell surface was(0.419±0.028)and there were significant statistical differences(t=7.193,P<0.001).There were significant differences in G 0/G 1,S-phase SK-MES-1 and NCI-H520 cells treated with DcR3 adapter on the target cell surface and the untreated blank control group(P<0.05),in which G 0/G 1 cells were significantly higher than untreated blank control group(P<0.05)and S-phase cells were significantly lower than untreated blank control group(P<0.05).The expression of FAS protein decreased when the SK-MES-1 and NCI-H520 cells were treated with DcR3 adapter on the surface of targeted cells,and it decreased with the concentration of DcR3 adapter on targeted cell surface increased(F=8.024,12.419,P<0.001).Conclusion DcR3 may be involved in the growth and proliferation of lung squamous cell carcinoma,which can provide a new target for its treatment.

关 键 词：肺鳞癌细胞 巨噬细胞迁移抑制因子 FAS蛋白 细胞增殖 细胞迁移 

分 类 号：R73[医药卫生—肿瘤]