十字花科植物DFR蛋白的生物信息学分析  

Bioinformatics Analysis of DFR Proteins in Cruciferae Plant

作　　者：胡燕[1] 尹明智[1] 匡光炼 黄杰 严秋香 HU Yan;YIN Ming-zhi;KUANG Guang-lian(College of Biology and Agricultural Science&Technology,Zunyi Normal University,Zunyi,Guizhou 563006)

机构地区：[1]遵义师范学院生物与农业科技学院

出　　处：《安徽农业科学》2019年第17期103-107,110,共6页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31501337);贵州省科学技术厅、遵义市科学技术局、遵义师范学院联合科技基金项目(黔科合LH字[2016]7014号);贵州省高层次创新型人才培养计划(遵市科合人才[2017] 11号);贵州省高等学校创新能力提升计划(黔教合协同创新字[2013]11)

摘　　要：采用生物信息学的方法分析已经在GenBank上注册十字花科植物的DFR基因序列及相应氨基酸序列,并对其理化性质、结构特征、系统进化关系等进行预测。结果表明,十字花科植物的DFR蛋白大部分都有6个外显子,分子质量36481.89~43129.21Da,大多数蛋白亚细胞定位于叶绿体中;除了BnaDFRA402,其他的DFR蛋白均具有酶活性部位、NADP结合位点和底物特异结合位点,这些保守区域形成了5个motifs;每个DFR蛋白均有多个磷酸化位点,以Ser为主,以Thr和Tyr磷酸化为辅;二级结构均由α-螺旋、β-转角、延伸链和无规则卷曲组成,其中α-螺旋为主要结构元件。The DFR gene sequences and corresponding amino acid sequences registered on GenBank in Cruciferae plants were analyzed by means of bioinformatics,their physical and chemical properties,structural characteristics and phylogenetic relationships were predicted.The results showed that most of DFR proteins in Cruciferae plants had six exons with molecular weight ranging from 36 481.89 Da to 43 129.21 Da.Most of the protein subcells were located in chloroplasts.Except BnaDFRA402,other DFR proteins had enzymatic activity sites,NADP binding sites and substrate specific binding sites,which formed five motifs.Each DFR protein had multiple phosphorylation sites,in which Ser was the dominant and Thr and Tyr were the minor.The secondary structures were composed of alpha helix,beta turn,extended strand and random coil,and alpha helix was the main structural element.

关 键 词：十字花科 花青素 DFR蛋白 生物信息学 

分 类 号：S188[农业科学—农业基础科学]