纳米化吉西他滨对Lewis肺癌细胞的体外毒性及凋亡的影响  

The Effect of Gemcitabine-loaded Solid-lipid Nanoparticles on the Cytotoxicity and Apoptosis of Lewis Lung Carcinoma Cells Cultured in vitro

作　　者：郑源强[1] 王彩霞[1] 石艳春[1] ZHENG Yuan-qiang;WANG Cai-xia;SHI Yan-chun(Inner Mongolia Key Laboratory of Molecular Biology,Inner Mongolia Medical University,Hohhot 010058,China)

机构地区：[1]内蒙古医科大学内蒙古自治区分子生物学重点实验室

出　　处：《内蒙古大学学报（自然科学版）》2019年第5期524-530,共7页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金(81871252,81660272,81460248);内蒙古自治区自然科学基金(2017ZD08,2014ZD05)资助项目

摘　　要：本研究旨在探讨新型吉西他滨硬脂酰脂质纳米颗粒(GemC18-NPs)对Lewis肺癌细胞(LLC细胞)的毒性及细胞凋亡的影响.不同纳米颗粒(GemC18、GemC18-NPs、空白纳米颗粒(NPs))和盐酸吉西他滨(GemHCl),分别处理体外培养的LLC细胞,MTT法检测药物对细胞的毒性,采用GraphPad Prism 5计算细胞存活率及IC50;流式细胞术检测细胞凋亡.结果表明GemC18-NPs对LLC细胞具有毒性并呈时间和浓度依赖性,同时具有缓释性;GemC18-NPs可有效诱导LLC细胞凋亡.To detect the cytotoxicity and apoptosis of a novel gemcitabine solid lipid nanoparticles formulation(gemcitabine nanoparticles,GemC18-NPs)on Lewis lung carcinoma cells(LLC),in vitro cultured LLC cells were treated with stearoyl gemcitabine(GemC18),gemcitabine hydrochloride(GemHCl),stearoyl gemcitabine nanoparticles(GemC18-NPs)or blank nanoparticles(NPs)respectively.The cytotoxicity of the GemC18-NPs were detected by MTT assay.The cell survival and IC50 values at each concentration were calculated and plotted as the log by GraphPad Prism 5 and the cell apoptosis rate was analyzed by flow cytometry.The results showed that,GemC18-NPs were cytotoxic to LLC cells in dose and time-dependent manner.The IC50 values of GemHCl treatment were significantly lower than those of GemC18-NPs or GemC18 treatment.While LLC cells were incubated with the GemC18-NPs for about 40 h,the percent of the dead LLC cells reached a similar level with GemHCl treatment for 24 h.Blank nanoparticles did not show significant cytotoxicity on the LLC cells.

关 键 词：吉西他滨 纳米颗粒 非小细胞肺癌 细胞毒性 

分 类 号：R734.2[医药卫生—肿瘤]